Journal of Southern Medical University ›› 2025, Vol. 45 ›› Issue (9): 1946-1958.doi: 10.12122/j.issn.1673-4254.2025.09.14
Qi YUN1,2(), Ruoli DU3,4, Yuying HE1,2, Yixin ZHANG5, Jiahui WANG3,4, Hongwei YE3,4, Zhenghong LI1,2, Qin GAO3,4(
)
Received:
2025-02-13
Online:
2025-09-20
Published:
2025-09-28
Contact:
Qin GAO
E-mail:yunqiyq1009@163.com;bbmcgq@126.com
Qi YUN, Ruoli DU, Yuying HE, Yixin ZHANG, Jiahui WANG, Hongwei YE, Zhenghong LI, Qin GAO. Cinnamic acid ameliorates doxorubicin-induced myocardial injury in mice by attenuating cardiomyocyte ferroptosis via inhibiting TLR4[J]. Journal of Southern Medical University, 2025, 45(9): 1946-1958.
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URL: https://www.j-smu.com/EN/10.12122/j.issn.1673-4254.2025.09.14
Fig.4 Visualization of molecular docking results. A: Potential interaction between CA and TLR4. B: Potential interaction between cinnamic acid and PTGS2.
Fig.5 Cardiac ultrasound images and cardiac function indexes of the mice in different groups (Mean±SD, n=6). A: Evaluation of cardiac function by M-mode echocardiography. B: Comparison of echocardiographic parameters. LVEF: Left ventricle ejection fraction; LVFS: Left ventricular fractional shortening. **P<0.01, ***P<0.001 vs the Sham group; #P<0.05, ##P<0.01, ###P<0.001 vs the DOX group.
Fig.8 Serum IL-6 (A) and TNF-α (B) levels in different groups (Mean±SD, n=6). ***P<0.001 vs the Sham group; #P<0.05, ##P<0.01, ###P<0.001 vs the DOX group.
Fig.9 Serum CK-MB (A), LDH (B) and MDA (C) levels in different groups (Mean±SD, n=6). ***P<0.001 vs the Sham group; #P<0.05, ##P<0.01, ###P<0.001 vs the DOX group.
Fig.10 TLR4, PTGS2, SLC7A11 and GPX4 protein expressions in the myocardial tissues in the 5 groups. A: Protein bands in Western blotting. B-E: Relative protein levels of TLR4, PTGS2, SLC7A11 and GPX4 proteins (Mean±SD, n=3). *P<0.05, **P<0.01, ***P<0.001 vs the Sham group; #P<0.05, ##P<0.01, ###P<0.001 vs the DOX group.
Fig.11 Comparison of TLR4, PTGS2, SLC7A11 and GPX4 protein expressions in the myocardial tissues among the 4 groups. A: Protein bands in Western blotting. B-E: Relative protein levels of TLR4, PTGS2, SLC7A11 and GPX4 proteins (Mean±SD, n=3). **P<0.01, ***P<0.001 vs the Sham group; #P<0.05, ##P<0.01 vs the DOX group.
Fig.12 Western blotting for detection of si-TLR4 transfection efficiency. A: Protein bands in Western blotting. B: Relative protein levels of TLR4 protein (Mean±SD, n=3). **P<0.01 vs the NC group.
Fig.13 Viability of HL-1 cells treated with different concentrations of DOX (A) and Erastin (B). Data are presented as Mean±SD (n=6). **P<0.01, ***P<0.001 vs the NC group.
Fig.14 ROS levels in HL-1 cardiomyocytes in each group. A:DCFH-DA staining of HL-1 of the cells (×200). B: Meanfluorescence intensity levels (Mean±SD, n=6) ***P<0.001 vs the NC group; #P<0.05 vs the DOX group; @<0.05 vs the Erastin group.
Fig.15 Immunofluorescence staining for GPX4 in HL-1cells in each group. A: Representative images ofimmunofluorescence staining of HL-1 cells in each group(×400). B: GPX4 fluorescence intensity in each group (Mean±SD,n=6). ***P<0.001vsP<0.01 vsthe NC group; ##P<0.01 vs the DOX group; @@P<0.01 vs the Erastin group.
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