图1 肌纤维E2信号对急性骨骼肌炎症的影响

Fig.1 Effect of E2 in myofiber signaling on acute skeletal myositis in mice. A: HE and immunofluorescence staining for detecting inflammation in the tibialis anterior muscle (TA) after CTX injury (Scale bar=50 μm). B: ELISA for detection of serum E2 in male and female mice at different time points after muscle injury (*P<0.05).

图2 急性骨骼肌炎中雌激素的受体表达

Fig.2 Expression of estrogen receptors in acute skeletal myositis. A: Western blotting of ERα and ERβ expression level in mature myotubes derived from C2C12 cells. B: qRT-PCR analysis of ERα and ERβ mRNA levels in the damaged muscle of female mice. C: Immunofluorescence staining of ERβ expression level in injured mouse muscle (Scale bar=50 μm). *P<0.05, **P<0.01.

图3 肌纤维E2信号对急性骨骼肌炎症损伤肌内单核巨噬细胞渗出影响

Fig.3 Effect of E2 signaling in myofibers on exudation of intramuscular mononuclear macrophages after acute skeletal muscle injury. A: ELISA for detecting serum E2 level in female mice after different treatments. B: ELISA for detecting serum E2 levels in female mice at different time points after OVX treatment. C: HE staining for detecting muscular inflammation in female mice with different treatments after CTX injury. D: Immunofluorescence staining for detecting muscular inflammation in female mice after CTX injury with different treatments. *P<0.05, **P<0.01; Scale bar=50 μm.

图4 肌纤维E2信号对急性骨骼肌炎症损伤肌内巨噬细胞表型影响

Fig.4 Effect of E2 signaling in myofibers on phenotype of intramuscular macrophages in mice after acute skeletal muscle injury. A: Flow cytometric analysis of mononuclear macrophages in the damaged muscle of female mice after OVX treatment. B: Flow cytometric analysis of the phenotype of macrophages in the injured muscles of female mice after OVX treatment. *P<0.05, **P<0.01.

图5 肌纤维E2信号对急性骨骼肌炎症损伤肌内炎症因子的影响

Fig.5 Effect of E2 signaling in myofibers on intramuscular inflammatory cytokines after acute skeletal muscle injury. A: qRT-PCR analysis of inflammatory cytokines in female mice after OVX treatment. B: qRT-PCR analysis of inflammatory cytokines in macrophages from the injured muscles isolated by flow cytometry in female mice after OVX treatment. *P<0.05, **P<0.01.

图6 肌纤维E2信号驱动急性骨骼肌炎症损伤肌内巨噬细胞胞葬作用

Fig.6 E2 signaling in myofibers drives intramuscular macrophage efferocytosis in acute skeletal myositis. A: Immunofluorescence detection of intramuscular macrophage efferocytosis in the damaged muscle. (scale bar=50 μm). B: Flow cytometry of intramuscular macrophage efferocytosis in the damaged muscle (*P<0.05).

图7 体外共培养体系中E2信号对巨噬细胞表型与胞葬作用的影响

Fig.7 Effect of E2 signaling on macrophage phenotypes and efferocytosis in an in vitro co-culture system. A: Immunofluorescence detection of macrophage phenotypes in the in vitro co-culture system. B: Immunofluorescence detection and flow cytometry analysis of macrophage efferocytosis in the in vitro co-culture system. *P<0.05; Scale bar=50 μm.

图8 肌纤维E2信号调控损伤骨骼肌内肌纤维再生修复

Fig.8 E2 signaling in myofibers regulates regeneration and repair of intramuscular myofibers in injured mouse skeletal muscles of female mice after different treatments (Immunofluorescence staining, scale bar=50 μm; *P<0.05).