图1 linc00467在肺腺癌细胞中的表达

Fig.1 Expression of LINC00467 in human bronchial epithelial cells 16HBE and lung adenocarcinoma cell lines A549 and H1299. ***P<0.001 vs 16HBE cells.

图2 linc00467在肺腺癌组织中的表达

Fig.2 Expression of LINC00467 in lung adenocarcinoma tissue based on data from TCGA database. ***P<0.001 vs Normal tissue.

图3 LINC00467与肺腺癌患者生存率和临床分期的关系

Fig.3 Correlation of LINC00467 expression level with survival rates (A) and clinical stages (B) of lung adenocarcinoma patients. *P<0.05 vs Stage I group; **P<0.01 vs Stage II group.

图4 A549和H1299细胞中linc00467的敲低效率

Fig.4 Interference efficiency of Shlinc00467 in A549 and H1299 cells. A: Transfection effect of Shlinc00467 (Original magnification: ×10); B: Quantification of the interference efficiency. **P<0.01 vs shNC group.

图5 敲低LINC00467对肺腺癌细胞增殖的影响

Fig.5 Effect of LINC00467 knockdown on proliferation of lung adenocarcinoma cells. A: Colony formation assay for assessing cell proliferation. B: Quantification of the colony number. **P<0.01 vs shNC group.

图6 敲低LINC00467对肺腺癌细胞转移的影响

Fig.6 Effect of LINC00467 knockdown on lung adenocarcinoma cell metastasis. A: Transwell assay for assessing cell migration and invasion (×10). B, C: Numbers of migrating and invading cells. ***P<0.001 vs shNC group.

图7 敲低LINC00467对肺腺癌细胞自噬和AMPK/mTOR信号通路的影响

Fig.7 Effects of LINC00467 knockdown on autophagy and AMPK/mTOR signaling pathways in lung adenocarcinoma cells. A: Immunofluorescence staining for detecting LC3 expression (×40). B, D: Western blotting of AMPK, p-AMPK, mTOR, p-mTOR, and LC3II/I proteins. C, E: Expression levels of p-AMPK/AMPK, p-mTOR/mTOR, LC3II/I, and p62. *P<0.05, **P<0.01 vs shNC group.

图8 肺腺癌组织与自噬通路关系的GSEA富集分析结果

Fig.8 GSEA enrichment analysis of the relationship between LINC00467 and autophagy pathway in lung adenocarcinoma tissue.

图9 自噬抑制剂对肺腺癌细胞自噬的影响

Fig.9 Effects of the autophagy inhibitor on autophagy of lung adenocarcinoma cells with LINC00467 knockdown. A: Immunofluorescence staining for LC3 expression (×40). B, C: Western blotting for detecting LC3II, LCI and p62 proteins. D-G: Quantification of LC3II/I and p62 protein levels.*P<0.05, **P<0.01, ***P<0.001 vs shNC group; #P<0.05, ##P<0.01 vs shlinc00467 group.

图10 LINC00467通过调控自噬影响肺腺癌细胞增殖

Fig.10 LINC00467 knockdown inhibits proliferation of lung adenocarcinoma cells by suppressing autophagy. A: Colony formation assay for evaluating cell proliferation. B: Quantification of the colony number. ***P<0.001 vs shNC group; ###P<0.01 vs shlinc00467 group.

图11 LINC00467通过调控自噬影响肺腺癌细胞转移

Fig.11 LINC00467 knockdown suppresses lung adenocarcinoma cell metastasis by regulating autophagy. A, C: Transwell assay for assessing cell migration and invasion (×10). B, D: Numbers of migrating and invading cells. **P<0.01 vs shNC group; #P<0.05, ##P<0.01, ###P<0.001 vs shlinc00467 group.

图12 LINC00467通过调控AMPK/mTOR信号通路影响肺腺癌细胞自噬

Fig.12 LINC00467 knockdown suppresses autophagy in lung adenocarcinoma cells by inhibiting the AMPK/mTOR signaling pathway. A,C: Western blotting for detecting AMPK, p-AMPK, mTOR, p-mTOR, LC3II, LC3I, and p62 proteins. B, D: Quantification of p-AMPK/AMPK, p-mTOR/mTOR, LC3II/I, and p62 protein levels. *P<0.05, **P<0.01 vs shNC group; #P<0.05 vs shlinc00467 group.

图13 LINC00467通过调控AMPK/mTOR信号通路影响肺腺癌细胞增殖

Fig.13 LINC00467 knockdown suppresses lung adenocarcinoma cell proliferation by inhibiting the AMPK/mTOR signaling pathway. A: Colony formation assay for assessing cell proliferation. B: Quantification of colony formation number. *P<0.05, **P<0.01, ***P<0.001 vs shNC group; #P<0.05, ##P<0.01 vs shlincC00467 group.

图14 LINC00467通过调控AMPK/mTOR信号通路影响肺腺癌细胞迁移

Fig.14 LINC00467 knockdown suppresses lung adenocarcinoma cell migration by inhibiting the AMPK/mTOR signaling pathway. A, C: Transwell assay for assessing cell migration and invasion (×10). B, D: Number of migrating and invading cells. *P<0.05, **P<0.01 vs shNC group; ##P<0.01 vs shlinc00467 group.