黄芩清热除痹胶囊通过PTEN/PI3K/AKT信号通路改善痛风性关节炎大鼠的炎症反应及尿酸、脂质代谢失衡

doi:10.12122/j.issn.1673-4254.2024.08.03

南方医科大学学报 ›› 2024, Vol. 44 ›› Issue (8): 1450-1458.doi: 10.12122/j.issn.1673-4254.2024.08.03

黄芩清热除痹胶囊通过PTEN/PI3K/AKT信号通路改善痛风性关节炎大鼠的炎症反应及尿酸、脂质代谢失衡

张先恒¹^,²(), 刘健¹^,³(), 韩琦¹^,², 陈一鸣¹^,², 丁香¹^,², 陈晓露²

^1.安徽中医药大学第一附属医院，安徽合肥 230011
^2.安徽中医药大学，安徽合肥 230012
^3.安徽省中医药科学院风湿病研究所，安徽合肥 230009

收稿日期:2024-04-28 出版日期:2024-08-20 发布日期:2024-09-06
通讯作者: 刘健 E-mail:1148465915@qq.com;liujianahzy@126.com
作者简介:张先恒，在读博士研究生，E-mail: 1148465915@qq.com
基金资助:
国家中医药管理局高水平中医药重点学科(ZYYZDXK-2023100);现代中医内科应用基础与开发研究安徽省重点实验室(2016080503B041);安徽省第12批“115”创新团队(皖人才办[2019]1号);安徽省名中医刘健工作室建设项目(中医药发展秘[2018] 11号)

Huangqin Qingrechubi Capsule alleviates inflammation and uric acid and lipid metabolism imbalance in rats with gouty arthritis by inhibiting the PTEN/PI3K/AKT signaling pathway

Xianheng ZHANG¹^,²(), Jian LIU¹^,³(), Qi HAN¹^,², Yiming CHEN¹^,², Xiang DING¹^,², Xiaolu CHEN²

^1.The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230011, China
^2.Anhui University of Chinese Medicine, Hefei 230012, China
^3.Institute of Rheumatology, Anhui Academy of Chinese Medicine, Hefei 230009, China

Received:2024-04-28 Online:2024-08-20 Published:2024-09-06
Contact: Jian LIU E-mail:1148465915@qq.com;liujianahzy@126.com

摘要/Abstract

摘要：

目的探究黄芩清热除痹胶囊（HQC）对痛风性关节炎（GA）大鼠炎症反应及尿酸、脂质代谢的影响，并探讨其可能机制。方法随机将60只SD大鼠分为6组：正常组，模型组，HQC低、中、高剂量组，秋水仙碱组（n=10）；以单钠尿酸盐注射右踝关节腔进行GA大鼠造模，HQC低、中、高剂量组及秋水仙碱组予相应剂量药物灌胃，模型组予等量生理盐水，连续7 d。造模后4、8、24、48、72 h检测各组大鼠足趾肿胀度；HE染色法进行滑膜组织学分析；ELISA检测血清中白细胞介素（IL）-10、IL-18、肿瘤坏死因子（TNF）-α、转化生长因子（TGF）-β1、脂联素（APN）、瘦素（LP）、抵抗素（RS）、内脂素（VF）的表达，检测滑膜中APN、LP、RS、VF的表达；生化法检测血清中高密度脂蛋白胆固醇（HDL-C）、甘油三酯（TG）、总胆固醇（TC）、血尿酸（BUA）的水平；RT-qPCR检测滑膜中磷酸酶与紧张素同源物（PTEN）、磷脂酰肌醇-3-激酶（PI3K）、蛋白激酶B（AKT） mRNA的表达；Western blotting检测PTEN、PI3K、p-PI3K、AKT、p-AKT 蛋白表达。结果与正常组比较，模型组足趾肿胀度升高（P<0.05），且在48 h达到高峰；HE染色显示大量炎性细胞浸润和滑膜组织增生；TNF-α、TGF-β1、IL-18、TC、TG、LP、RS、VF、BUA、p-PI3K、p-AKT的表达增加（P<0.05），IL-10、APN、HDL-C、PTEN的表达降低（P<0.05）。与模型组比较，HQC和秋水仙碱可降低TNF-α、TGF-β1、IL-18、TC、TG、LP、RS、VF、BUA、p-PI3K、p-AKT的表达（P<0.05），升高IL-10、APN、HDL-C、PTEN的表达（P<0.05），减轻滑膜组织病理损伤，改善足趾肿胀度。结论 HQC可改善GA大鼠炎症反应及尿酸、脂质代谢失衡，可能与抑制PTEN/PI3K/AKT信号通路有关。

关键词: 痛风性关节炎, 黄芩清热除痹胶囊, 炎症, 尿酸代谢, 脂质代谢, PTEN/PI3K/AKT信号通路

Abstract:

Objective To investigate the effects of Huangqin Qingrechubi Capsule (HQC) on inflammation and uric acid and lipid metabolism in rats with gouty arthritis (GA) and its mechanism. Methods SD rat models of GA established by injecting monosodium urate into the right ankle joint were treated with saline, colchicine and HQC at low, medium and high doses (n=10) by gavage for 7 days. Toe swelling of the rats was detected at 4, 8, 24, 48 and 72 h after modeling, and synovial histological changes were observed with HE staining. Serum levels of interleukin-10 (IL-10), IL-18, tumor necrosis factor‑α (TNF‑α), transforming growth factor-β1 (TGF-β1), adiponectin, leptin, resistin and visfatin were measured by ELISA, and the levels of high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), total cholesterol (TC), and uric acid (BUA) were detected. RT-qPCR and Western blotting were used to detect the mRNA expressions of phosphatase and tensin homolog (PTEN), phosphatidylinositol-3-kinase (PI3K) and protein kinase B (AKT) and the protein expressions of PTEN, PI3K, p-PI3K, AKT and p-AKT. Results The rat models of GA showed obvious toe swelling, which reached the peak level at 48 h. HE staining revealed massive inflammatory cell infiltration and synovial tissue hyperplasia. The rat models showed significantly increased expressions of TNF-α, TGF-β1, IL-18, TC, TG, leptin, resistin and visfatin, BUA, p-PI3K, and p-AKT and lowered levels of IL-10, APN, HDL-C, and PTEN. Treatment with HQC and colchicine obviously improved these changes and alleviated synovial pathologies and toe swelling in the rat models. Conclusion HQC can improve inflammation and correct the imbalance of uric acid and lipid metabolism in GA rats possibly by inhibiting the PTEN/PI3K/AKT signaling pathway.

Key words: gouty arthritis, Huangqin Qingrechubi Capsule, inflammation, uric acid metabolism, lipid metabolism, PTEN/PI3K/AKT signaling pathway

张先恒, 刘健, 韩琦, 陈一鸣, 丁香, 陈晓露. 黄芩清热除痹胶囊通过PTEN/PI3K/AKT信号通路改善痛风性关节炎大鼠的炎症反应及尿酸、脂质代谢失衡[J]. 南方医科大学学报, 2024, 44(8): 1450-1458.

Xianheng ZHANG, Jian LIU, Qi HAN, Yiming CHEN, Xiang DING, Xiaolu CHEN. Huangqin Qingrechubi Capsule alleviates inflammation and uric acid and lipid metabolism imbalance in rats with gouty arthritis by inhibiting the PTEN/PI3K/AKT signaling pathway[J]. Journal of Southern Medical University, 2024, 44(8): 1450-1458.

图/表 8

表1 引物设计表

Tab.1 Primer design table

Gene	Amplicon size	Primer sequence (5'→3')
PTEN	140	F:TATCTTGTGCTCACCCTGAC R:CTGCTAGCCTCTGGATTTGA
PI3K	123	F:CGAAACAAAGCCGAGAACCT R:GACGCAATGTTTGACTTCGC
AKT	143	F:CAGGTTCACCCAGTGACAAC R:CTCCTTCACCAGGATCACCT
β-actin	150	F:CCCATCTATGAGGGTTACGC R:TTTAATGTCACGCACGATTTC

表2 抗体信息

Tab.2 Antibody information

Antibody	Dilution rate	Species
PI3K	1:1000	Rabbit
p-PI3K	1:5000	Mouse
AKT	1:1000	Rabbit
p-AKT	1:1000	Rabbit
PTEN	1:1000	Rabbit
GAPDH	1:2000	Mouse

图1 注射MSU 48 h后各组大鼠右踝关节图像

Fig.1 Right ankle joints of the rats in each group after MSU injection 48 h. A: Normal control group; B: Model group; C: HQC-L group; D: HQC-M group; E: HQC-H group; F: Colchicine group.

表3 HQC对GA大鼠足趾肿胀度的影响

Tab.3 Effect of HQC on toe swelling in GA rats (Mean±SD, mL, n=10)

Groups	4 h	8 h	24 h	48 h	72 h
Normal	1.856±0.025	1.796±0.023	1.702±0.027	1.650±0.025	1.645±0.029
Model	2.015±0.073*	2.156±0.045*	2.335±0.058*	2.547±0.059*	2.456±0.068*
HQC-L	1.945±0.059^△#	2.096±0.045^△#	2.170±0.059^△#	1.954±0.057^△#	1.741±0.065^△#
HQC-M	1.908±0.033^△#	1.995±0.036^△#	1.896±0.026^△#	1.805±0.040^△#	1.693±0.040^△#
HQC-H	1.896±0.038^△#	1.920±0.035^△	1.855±0.030^△	1.727±0.040^△	1.642±0.034^△
Colchicine	1.853±0.036^△	1.914±0.040^△	1.857±0.047^△	1.714±0.063^△	1.638±0.030^△

图2 各组大鼠滑膜组织HE染色图像

Fig.2 HE staining of synovial tissue of the rats in each group. Arrows indicate infiltration of inflammatory cells. A: Normal control group; B: Model group; C: HQC-L group; D: HQC-M group; E: HQC-H group; F: Colchicine group.

图3 各组大鼠TNF-α、TGF-β1、IL-18、IL-10表达比较

Fig.3 Comparison of TNF-α, TGF-β1, IL-18 and IL-10 expressions among the groups. *P<0.05 vs A; △P<0.05 vs B; #P<0.05 vs F. A: Normal control group; B: Model group; C: HQC-L group; D: HQC-M group; E: HQC-H group; F: Colchicine group.

图4 各组大鼠TC、TG、HDL-C、APN、LP、RS、VF、BUA水平比较

Fig.4 Comparison of TC, TG, HDL-C, APN, LP, RS, VF and BUA levels among the groups. *P<0.05 vs A; △P<0.05 vs B; #P<0.05 vs F. A: Normal control group; B: Model group; C: HQC-L group; D: HQC-M group; E: HQC-H group; F: Colchicine group.

图5 各组大鼠PTEN、PI3K、AKT mRNA表达比较

Fig.5 Comparison of mRNA expressions of PTEN, PI3K and AKT among the groups. *P<0.05 vs A; △P<0.05 vs B; #P<0.05 vs F. A: Normal control group; B: Model group; C: HQC-L group; D: HQC-M group; E: HQC-H group; F: Colchicine group.

参考文献 45

1	Neogi T, Jansen TL, Dalbeth N, et al. 2015 Gout classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative[J]. Ann Rheum Dis, 2015, 74(10): 1789-98.
2	Yu XH, Wang T, Huang SP, et al. Evaluation of the causal effects of blood lipid levels on gout with summary level GWAS data: two-sample Mendelian randomization and mediation analysis[J]. J Hum Genet, 2021, 66(5): 465-73.
3	张先恒, 刘健, 周琴, 等. 痛风性关节炎患者脂代谢变化及其与免疫、炎症指标和血尿酸的相关性分析[J]. 风湿病与关节炎, 2021, 10(8): 1-5.
4	朱子文, 李琳, 李晓玲, 等. 胆固醇/三酰甘油水平升高对痛风发病频率的影响[J]. 蚌埠医学院学报, 2022, 47(12): 1639-42, 1646.
5	Suh YS, Noh HS, Kim HJ, et al. Differences in clinical and dietary characteristics, serum adipokine levels, and metabolomic profiles between early- and late-onset gout[J]. Metabolites, 2021, 11(6): 399.
6	Liang WD, Chen LT, Cheng XC, et al. Analysis of the relationship of refractory gout between potential biomarkers and diet structure and lifestyle based on ¹H-NMR[J]. J Orthop Surg Res, 2024, 19(1): 78.
7	王颖, 高惠英. 脂联素介导免疫炎症机制在常见风湿性疾病中的研究进展[J]. 临床医药实践, 2023, 32(7): 517-21, 526.
8	郑戈, 毕兵, 朱晶, 等.血清缺氧诱导因子-1α、Dickkopf-1、抵抗素与痛风性关节炎疼痛、骨破坏的相关性及临床意义[J].安徽医药,2022, 26(11): 2280-4.
9	李中南, 周媛媛, 邢宇婷, 等. 萆苓祛痛方对糖尿病痛风大鼠心肌组织内脂素蛋白表达干预及对心脏病理影响[J]. 辽宁中医药大学学报, 2020, 22(4): 17-20.
10	汪元, 刘健, 黄传兵, 等. 中药内服外敷治疗湿热瘀阻型痛风性关节炎急性发作30例临床观察[J]. 中医杂志, 2014, 55(15): 1299-302.
11	张先恒, 刘健, 周琴, 等. 基于数据挖掘分析黄芩清热除痹胶囊对痛风合并高脂血症患者生化检测指标的影响[J]. 湖南中医药大学学报, 2021, 41(9): 1389-94.
12	孙广瀚, 刘健, 万磊, 等. 黄芩清热除痹胶囊含药血清对痛风性关节炎CD4⁺T细胞与心肌细胞共培养后miR-23a-3p/PTEN表达的影响[J]. 北京中医药大学学报, 2021, 44(8): 735-43.
13	Zhang XH, Liu J, Sun YQ, et al. Chinese herbal compound Huangqin Qingrechubi capsule reduces lipid metabolism disorder and inflammatory response in gouty arthritis via the LncRNA H19/APN/PI3K/AKT cascade[J]. Pharm Biol, 2023, 61(1): 541-55.
14	Sun K, Luo J, Guo J, et al. The PI3K/AKT/mTOR signaling pathway in osteoarthritis: a narrative review[J]. Osteoarthritis Cartilage, 2020, 28(4): 400-9.
15	Zhou P, Meng XW, Nie ZM, et al. PTEN: an emerging target in rheumatoid arthritis?[J]. Cell Commun Signal, 2024, 22(1): 246.
16	Liu YC, Han YQ, Liu YQ, et al. Xanthoceras sorbifolium leaves alleviate hyperuricemic nephropathy by inhibiting the PI3K/AKT signaling pathway to regulate uric acid transport[J]. J Ethnopharmacol, 2024, 327: 117946.
17	Zhang MQ, Sun KX, Guo X, et al. The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway[J]. J Ethnopharmacol, 2023, 317: 116770.
18	Kwon M, Kim Y, Lee J, et al. Neohesperidin dihydrochalcone and neohesperidin dihydrochalcone-O-glycoside attenuate subcutaneous fat and lipid accumulation by regulating PI3K/AKT/mTOR pathway in vivo and in vitro [J]. Nutrients, 2022, 14(5): 1087.
19	Xu YJ, Wei RS, Li XH, et al. MiR-421 promotes lipid metabolism by targeting PTEN via activating PI3K/AKT/mTOR pathway in non-small cell lung cancer[J]. Epigenomics, 2022,79: 1305-17.
20	Coderre TJ, Wall PD. Ankle joint urate arthritis in rats provides a useful tool for the evaluation of analgesic and anti-arthritic agents[J]. Pharmacol Biochem Behav, 1988, 29(3): 461-6.
21	蔡唐彦, 王旭, 何浈, 等. 急性痛风性关节炎大鼠模型的建立及模型维持时间观察[J]. 中国实验动物学报, 2017, 25(5): 494-9.
22	Zhou WW, Wang YT, Huang YR, et al. Huangqin Qingre Qubi Capsule inhibits RA pathology by binding FZD8 and further inhibiting the activity of Wnt/β-catenin signaling pathway[J]. J Ethnopharmacol, 2023, 302(Pt A): 115886.
23	Sandoval-Plata G, Nakafero G, Chakravorty M, et al. Association between serum urate, gout and comorbidities: a case-control study using data from the UK Biobank[J]. Rheumatology, 2021, 60(7): 3243-51.
24	Ran ZJ, Xue XM, Han L, et al. Decrease in serum urate level is associated with loss of visceral fat in male gout patients[J]. Front Endocrinol, 2021, 12: 724822.
25	Fang YJ, Wu TY, Lin CL, et al. Effects of urate-lowering therapy on risk of hyperlipidemia in gout by a population-based cohort study and on in vitro hepatic lipogenesis-related gene expression[J]. Mediators Inflamm, 2020, 2020: 8890300.
26	Wu J, Zhang YP, Qu Y, et al. Efficacy of uric acid-lowering therapy on hypercholesterolemia and hypertriglyceridemia in gouty patients[J]. Int J Rheum Dis, 2019, 22(8): 1445-51.
27	Zhang J, Ji XP, Dong ZH, et al. Impact of fenofibrate therapy on serum uric acid concentrations: a review and meta-analysis[J]. Endocr J, 2021, 68(7): 829-37.
28	龚发萍, 郑鸣. 黄芩的化学成分及药理作用[J]. 临床合理用药杂志, 2021, 14(34): 176-8.
29	张嘉豪, 呼田, 周雪薇, 等.栀子药理作用及临床应用研究进展[J].辽宁中医药大学学报, 2024, 26(5): 93-8.
30	李伟, 徐伟. 黄芩苷药理作用研究进展[J]. 中西医结合研究, 2022, 14(3): 193-6.
31	曹玲, 崔琳琳, 孙艳, 等. 威灵仙的药理作用及其机制研究进展[J]. 药物评价研究, 2022, 45(11): 2364-70.
32	吴静雨, 陈晓凡, 徐万爱, 等. 薏苡仁活性成分研究进展[J]. 中国中药杂志, 2024, 49(6): 1474-84.
33	王文娟, 王海明, 王胜军, 等. 苦杏仁苷对骨质疏松大鼠骨折愈合的影响[J]. 中国临床药理学杂志, 2024, 40(8): 1198-202.
34	Hao GF, Xu XF, Song JY, et al. Lipidomics analysis facilitate insight into the molecular mechanisms of urate nephropathy in a gout model induced by combination of MSU crystals injection and high-fat diet feeding[J]. Front Mol Biosci, 2023, 10: 1190683.
35	Spartalis M, Spartalis E, Tzatzaki E, et al. The beneficial therapy with colchicine for atherosclerosis via anti-inflammation and decrease in hypertriglyceridemia[J]. Cardiovasc Hematol Agents Med Chem, 2018, 16(2): 74-80.
36	Mylonas KS, Kapelouzou A, Spartalis M, et al. KLF4 upregulation in atherosclerotic thoracic aortas: exploring the protective effect of colchicine-based regimens in a hyperlipidemic rabbit model[J]. Ann Vasc Surg, 2022, 78: 328-35.
37	Lee SJ, Nam WD, Na HJ, et al. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation[J]. Biochem Pharmacol, 2008, 76(1): 79-90.
38	Zhang D, Li L, Li J, et al. Colchicine improves severe acute pancreatitis-induced acute lung injury by suppressing inflammation, apoptosis and oxidative stress in rats[J]. Biomedecine Pharmacother, 2022, 153: 113461.
39	Liu PY, Xu Y, Ye JX, et al. Qingre Huazhuo Jiangsuan Decoction promotes autophagy by inhibiting PI3K/AKT/mTOR signaling pathway to relieve acute gouty arthritis[J]. J Ethnopharmacol, 2023, 302(Pt A): 115875.
40	Tavares LD, Galvão I, Costa VV, et al. Phosphoinositide-3 kinase gamma regulates caspase-1 activation and leukocyte recruitment in acute murine gout[J]. J Leukoc Biol, 2019, 106(3): 619-29.
41	Liu L, Zhu XX, Zhao TY, et al. Sirt1 ameliorates monosodium urate crystal-induced inflammation by altering macrophage polarization via the PI3K/Akt/STAT6 pathway[J]. Rheumatology, 2019, 58(9): 1674-83.
42	Yang BD, Xin ML, Liang SF, et al. Naringenin ameliorates hyperuricemia by regulating renal uric acid excretion via the PI3K/AKT signaling pathway and renal inflammation through the NF-κB signaling pathway[J]. J Agric Food Chem, 2023, 71(3): 1434-46.
43	甘斌, 李华南, 李松, 等. 基于脂代谢和炎症反应探讨两种湿热证痛风性关节炎大鼠模型的构建[J]. 中国比较医学杂志, 2023, 33(1): 26-33.
44	Lyu S, Ding RW, Liu P, et al. LC-MS analysis of serum for the metabolomic investigation of the effects of pulchinenoside b4 administration in monosodium urate crystal-induced gouty arthritis rat model[J]. Molecules, 2019, 24(17): 3161.
45	Yang Y, Xian W, Wu DD, et al. The role of obesity, type 2 diabetes, and metabolic factors in gout: a Mendelian randomization study[J]. Front Endocrinol, 2022, 13: 917056.

[1]	范正媛, 沈子涵, 李亚, 沈婷婷, 李高峰, 李素云. 补肺益肾方对香烟烟雾提取物诱导的人支气管上皮细胞损伤的保护作用及其机制[J]. 南方医科大学学报, 2025, 45(7): 1372-1379.
[2]	王立明, 陈宏睿, 杜燕, 赵鹏, 王玉洁, 田燕歌, 刘新光, 李建生. 益气滋肾方通过抑制PI3K/Akt/NF-κB通路改善小鼠慢性阻塞性肺疾病的炎症反应[J]. 南方医科大学学报, 2025, 45(7): 1409-1422.
[3]	夏冰, 彭进, 丁九阳, 王杰, 唐国伟, 刘国杰, 王沄, 万昌武, 乐翠云. ATF3通过NF-κB信号通路调控动脉粥样硬化斑块内的炎症反应[J]. 南方医科大学学报, 2025, 45(6): 1131-1142.
[4]	王心恒, 邵小涵, 李童童, 张璐, 杨勤军, 叶卫东, 童佳兵, 李泽庚, 方向明. 平喘宁方通过调控HMGB1/Beclin-1轴介导的自噬改善患寒哮证大鼠的气道炎症[J]. 南方医科大学学报, 2025, 45(6): 1153-1162.
[5]	牛民主, 殷丽霞, 乔通, 尹林, 张可妮, 胡建国, 宋传旺, 耿志军, 李静. 旱莲苷A通过调控JAK2/STAT3通路抑制M1型巨噬细胞极化改善葡聚糖硫酸钠诱导的小鼠结肠炎[J]. 南方医科大学学报, 2025, 45(6): 1297-1306.
[6]	李丹丹, 楚佳鑫, 闫妍, 徐文隽, 朱行春, 孙韵, 丁浩峰, 任丽, 朱博. 姜黄素通过下调HIF-1α通路抑制非小细胞肺癌脂质代谢[J]. 南方医科大学学报, 2025, 45(5): 1039-1046.
[7]	杨洋, 王凯, 柳鉴修, 周志谟, 贾雯, 吴思谋, 李金星, 何方, 程如越. 生命早期两歧双歧杆菌BD-1干预可缓解注意缺陷多动障碍雌性大鼠幼年期的多动行为[J]. 南方医科大学学报, 2025, 45(4): 702-710.
[8]	朱正望, 王琳琳, 赵静涵, 马瑞雪, 余雨春, 蔡庆春, 王兵, 朱平生, 苗明三. 退黄合剂通过调控法尼醇X受体抑制NLRP3炎症小体改善α-萘异硫氰酸酯诱导的大鼠胆汁淤积[J]. 南方医科大学学报, 2025, 45(4): 718-724.
[9]	储菲, 陈孝华, 宋博文, 杨晶晶, 左芦根. 苏荠宁黄酮通过抑制PI3K/AKT信号通路拮抗肠上皮细胞凋亡改善小鼠实验性结肠炎[J]. 南方医科大学学报, 2025, 45(4): 819-828.
[10]	张金水, 李硕, 魏栋栋, 成昕, 邓云, 张有志. 石墨烯发热膜热疗改善小鼠冻伤的作用及机制[J]. 南方医科大学学报, 2025, 45(3): 522-530.
[11]	曹周芳, 汪元, 王梦娜, 孙玥, 刘菲菲. LINC00837/miR-671-5p/SERPINE2功能轴促进类风湿关节炎成纤维细胞样滑膜细胞的恶性病理学过程[J]. 南方医科大学学报, 2025, 45(2): 371-378.
[12]	罗维, 王宇航, 刘延松, 王媛媛, 艾磊. 高糖环境通过抑制免疫反应基因1的表达诱导巨噬细胞促炎性M1型极化[J]. 南方医科大学学报, 2025, 45(1): 1-9.
[13]	张玉如, 万磊, 方昊翔, 李方泽, 王丽文, 李柯霏, 闫佩文, 姜辉. miR-155-5p介导PIK3R1负调控PI3K/AKT信号通路促进原发性干燥综合征人唾液腺上皮细胞增殖[J]. 南方医科大学学报, 2025, 45(1): 65-71.
[14]	左涵珺, 段兆达, 王朝, 郭涛, 石金沙, 石浩龙, 李娟娟. 天麻素经PI3K/AKT通路改善新生大鼠缺氧缺血性脑损伤后小胶质细胞介导的炎症反应[J]. 南方医科大学学报, 2024, 44(9): 1712-1719.
[15]	李明远, 张玮, 华梦晴. 甲基巴多索龙通过抑制NLRP3炎症小体活化缓解小鼠急性肝损伤[J]. 南方医科大学学报, 2024, 44(9): 1662-1669.

黄芩清热除痹胶囊通过PTEN/PI3K/AKT信号通路改善痛风性关节炎大鼠的炎症反应及尿酸、脂质代谢失衡

Huangqin Qingrechubi Capsule alleviates inflammation and uric acid and lipid metabolism imbalance in rats with gouty arthritis by inhibiting the PTEN/PI3K/AKT signaling pathway

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 45

相关文章 15

编辑推荐

Metrics

本文评价