南方医科大学学报

南方医科大学学报 ›› 2026, Vol. 46 ›› Issue (4): 899-906.doi: 10.12122/j.issn.1673-4254.2026.04.18

• • 上一篇    

电针“百会”“足三里”通过激活海马星形胶质细胞改善单次长时间应激小鼠恐惧记忆消退障碍

陈潇煜1(), 刘畅1, 杨绍杰1, 王娟2, 程平2, 尹红宇1, 王婧吉1(), 朱国旗2()   

  1. 1.安徽中医药大学第二附属医院，安徽 合肥 230061
    2.安徽中医药大学分子生物学（脑病）重点实验室，安徽 合肥 230012
  • 收稿日期:2025-06-06 出版日期:2026-04-20 发布日期:2026-04-24
  • 通讯作者: 王婧吉,朱国旗 E-mail:cxy199906@163.com;wangjingji@ahtcm.edu.cn;Guoqizhu@gmail.com
  • 作者简介:陈潇煜，在读硕士研究生，E-mail: cxy199906@163.com
  • 基金资助:
    国家自然科学基金(82404890);安徽省临床医学研究转化专项(202304295107020105);安徽省高等学校科学研究项目(2024AH040137);安徽省高等学校科学研究项目(2024AH051044);新安医学与中医药现代化研究所“揭榜挂帅”项目(2023CXMMTCM013)

Electroacupuncture at Baihui and Zusanli improves fear memory extinction in single prolonged stress mice by activating hippocampal astrocytes

Xiaoyu CHEN1(), Chang LIU1, Shaojie YANG1, Juan WANG2, Ping CHENG2, Hongyu YIN1, Jingji WANG1(), Guoqi ZHU2()   

  1. 1.Second Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230061, China
    2.Key Laboratory of Molecular Biology/Brain diseases of Anhui University of Chinese Medicine, Hefei 230012, China
  • Received:2025-06-06 Online:2026-04-20 Published:2026-04-24
  • Contact: Jingji WANG, Guoqi ZHU E-mail:cxy199906@163.com;wangjingji@ahtcm.edu.cn;Guoqizhu@gmail.com
  • Supported by:
    National Natural Science Foundation of China(82404890)

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摘要：

目的 探究电针对单次长时间应激（SPS）小鼠恐惧记忆消退的影响及星形胶质细胞（AST）参与的潜在机制。 方法 将36只雄性C57BL/6J小鼠随机分为6组（6只/组）：实验一分为对照组、模型（SPS）组、电针治疗（EA）组和帕罗西汀阳性药（PRX）组；实验二分为Saline组和CNO组，通过化学遗传病毒抑制小鼠海马AST。除对照组外，其余各组采用SPS法进行模型制备。造模7 d后，EA、Saline和CNO组取“百会”“足三里”进行电针干预，PRX组予帕罗西汀溶液灌胃，连续干预10 d。采用条件性恐惧实验和高架十字迷宫实验评价小鼠恐惧记忆消退和焦虑样行为，其中CNO组在检测开始前30 min予CNO腹腔注射；采用Western blotting检测小鼠海马GFAP和CX43的蛋白表达。 结果 与对照组比较，SPS组小鼠恐惧再暴露阶段3~15 min内的凝滞时间、消退测试阶段的凝滞时间增加（P<0.05），恐惧消退指数、进入开放臂的时间降低（P<0.01），GFAP的平均荧光强度和AST的分支数量在距离胞体40~60 μm降低（P<0.05），海马GFAP和CX43蛋白的表达降低（P<0.05）。与SPS组比较，EA组与PRX组小鼠恐惧再暴露阶段3~6 min内的凝滞时间、消退测试阶段的凝滞时间降低（P<0.05），恐惧消退指数、进入开放臂的时间增加（P<0.05），GFAP的平均荧光强度增加（P<0.001），海马CX43蛋白的表达增加（P<0.05）；EA组海马AST的分支数量在距离胞体50 μm增加（P<0.05）。与Saline组比较，CNO组小鼠恐惧再暴露阶段3~9 min内和消退测试阶段的凝滞时间增加（P<0.05），恐惧消退指数和进入开放臂的时间降低（P<0.05）。 结论 电针“百会”“足三里”可通过激活海马AST改善SPS小鼠的恐惧记忆消退障碍和焦虑样行为。

关键词: 创伤后应激障碍, 电针, 恐惧记忆消退, 星形胶质细胞

Abstract:

Objective To explore the effect of electroacupuncture (EA) on fear memory extinction in single prolonged stress (SPS) mice and the potential mechanism involving astrocytes. Methods Thirty-six male C57BL/6J mice were randomly divided into the control, SPS model, EA treatment, paroxetine positive drug (PRX), Saline and CNO group. In Saline and CNO group, chemical genetic virus was used to inhibit the hippocampal astrocytes. Except for control group, the other groups were subjected to SPS modeling. After 7 days, the EA, Saline and CNO group were treated with EA at Baihui and Zusanli, while PRX group was given paroxetine solution for 10 days. Fear conditioning test and elevated plus maze test were used to evaluate fear memory extinction and anxiety-like behaviors, and the CNO group was administered CNO 30 minutes before the tests start. Immunofluorescence staining was used to observe the changes in the number and morphology of hippocampal astrocytes, and hippocampal expressions of GFAP and CX43 proteins were detected using Western blotting. Results In the mouse models with SPS, EA intervention significantly reduced the freezing time during fear re-exposure for 3-15 min and in the fear extinction phase. EA intervention also significantly increased the extinction coefficient, time spent in the open arms, the mean fluorescence intensity of GFAP, the number of astrocyte branches 50 μm from the soma, and the expression of CX43 proteins in the hippocampus of SPS mice. Conclusion EA at Baihui and Zusanli improves fear memory extinction and anxiety-like behaviors in SPS mice by activating hippocampal astrocytes.

Key words: post-traumatic stress disorder, electroacupuncture, fear memory extinction, astrocytes