Abstract: Objective To detect the expression of transient receptor potential canonical 1 (TRPC1) in a mouse model of
ozone-induced lung inflammation and explore its role in lung inflammation. Methods In a mouse model of lung inflammation
established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT-PCR, Wstern
blotting and immunohistochemistry. Results Compared to the control mice, the mice exposed to ozone showed significantly
increased expression level of TRPC1 mRNA and protein in the inflammatory lung tissues (P<0.05). Immunohistochemistry
showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells
in the inflammatory lung tissues (P<0.05). The mRNA and protein expression levels of TRPC1 were positively correlated with
the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed
mice (P<0.01). Conclusion TRPC1 may play a role in ozone-induced lung inflammation in mice.