Abstract: Objective To explore the effects on cell proliferation and invasion as well as molecular basis after suppressing EZH2
expression in endometrial carcinoma cells by using siRNAs. Methods RT-PCR was used to examine the expression of EZH2 in
endometrial carcinoma and their paracancerous tissues. SiRNAs targeting to EZH2 were transfected to endometrial carcinoma
cells, and MTT, FACS, and boyden assays were utilized to examine cell proliferation, cell cycle change, and cell invasion.
Finally, the molecular mechanisms of EZH2 on cell function alteration were investigated. Results Compared with
paracancerous tissues, increased expression trend of EZH2 mRNA was showed in endometrial carcinoma tissues. Further,
knocking down EZH2 expression inhibited cell growth, cell cycle transition from G1 to S phase, and cell invasion ability.
Molecular basis indicated that suppression of EZH2 downregulated the expression of E2F1 and MMP9 and upregulated tumor
suppressor p21 expression. Conclusions EZH2 expression is increased in endometrial carcinoma tissues. Knocking down
EZH2 expression suppresses the cell growth, cell cycle transition and cell invasion by downregulated E2F1 and MMP9, and
upregulated tumor suppressor p21 expression.