Abstract: ObjectiveTo investigate the expression of adiponectin and its receptors (AdipoRs) in the retina of normal mice and
mice with type1 diabetes mellitus (T1DM).MethodsC57BL/6 mice were randomly divided into control group and
streptozotocin-induced T1DM group. Two months after the modeling, the total protein and adiponectin protein expression in
the retina and choroid were measured using BCA method and enzyme-linked immunosorbent assay, respectively. Quantitative
RT-PCR was performed to detect the mRNA expressions of AdipoRs in the retina and choroid, and Western blotting was
employed to examine the protein expressions of AdipoRs in the retina.ResultsAdiponectin and AdipoRs proteins were
expressed in the retina and choroid in normal mice. The expressions of adiponectin and AdipoR1were up-regulated in the
retina of mice with T1DM while AdipoR2expression exhibited no significant changes.ConclusionAdiponectin and AdipoR1
may play an important role in the evolvement of type1diabetic retinopathy.