Abstract:
Objective To explore the role of annexin A2(ANXA2) expression in the intestinal mucosa in the pathogenesis of
inflammatory bowel disease (IBD).MethodsIntestinal or colonic mucosal biopsy samples were obtained from54patients with
ulcerative colitis (UC),37with Crohn’s disease (CD), and15healthy control subjects. Immunohistochemistry was employed to
examine the expression of ANXA2in the intestinal mucosa, and mRNA expression of ANXA2was detected using real-time
PCR.ResultsImmunohistochemistry showed a ANXA2positivity rate of83.3% (45/54) in patients with UC, 27.0% (10/37) in
patients with CD, and53.3% (8/15) in the control subjects. ANXA2 expression in the intestinal or colonic mucosa was
significantly up-regulated in patients with UC compared with the patients with CD and healthy control subjects, but was
significantly lower in patients with CD than in the healthy controls (P<0.05). The expression levels of ANXA2were strongly
associated with the severity of clinical manifestations and the histopathological grades of UC (P<0.05). Compared with the
healthy controls and patients with CD, patients with UC showed a significantly increased ANXA2mRNA expression level in
the inflamed mucosa of UC (P<0.05). ConclusionANXA2can serve as a marker for differential diagnosis of IBD, and its
up-regulated expression is closely related to the pathogenesis of UC.