Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (11): 1633-1636.

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Effect of calcineurin Aα gene overexpression on the myocardium apoptosis induced by hypoxia-reoxygenation and adrenergic receptors

SHEN Xiao-mei1,2,ZHANG Ju-yan2,CHENG Bei1 1Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;2First Affiliated Hospital,Shanxi Medical University,Taiyuan 030001,China   

  1. 华中科技大学同济医学院协和医院心血管内科; 山西医科大学第一附属医院; 华中科技大学同济医学院协和医院心血管内科 湖北武汉430022山西医科大学第一附属医院; 山西太原030001; 湖北武汉430022;
  • Online:2006-11-20 Published:2006-11-20

Abstract: Objective To investigate the effect of calcineurin AαcDNA(AdCnAα) overexpression as a result of adenovirally mediated gene transfer on neonatal rat cardiac myocyte apoptosis induced by hypoxia-reoxygenation(H/R) and adrenergic receptors.Methods Neonatal rat cardiac myocytes were cultured for 20 h after AdCnAα transfection,and treated with isoproterenol(10 μmol/L) and 24 h of hypoxia followed by 4 h of reoxygenation(24H/4R).The cardiac myocyte apoptosis induced by the treatments was assessed by flow cytometry and DNA laddering,and the levels of calcineurin,p38 and phosphorylation p38(p-p38) were determined by Western blotting and(or) RT-PCR.Results AdCnAα transfection promoted cultured neonatal rat cardiac myocyte apoptosis induced by isoproterenol+24H/4R as compared with the treated cells without transfection(14.247±0.525 vs 10.763±1.554,P<0.01),along with greater phosphorylation p38 protein expression(1.60±0.22 vs 2.42±0.19,P<0.01).The levels of p38 underwent no obvious change after AdCnAα transfection(P<0.05).Conclusions AdCnAα transfection can promote cardiac myocyte apoptosis induced by H/R and adrenergic receptors,the mechanism of which might be associated with p38 mitongen-activated protein kinase(p38MAPK) activation. 

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