public javax.script.ScriptEngineManager() public javax.script.ScriptEngineManager(java.lang.ClassLoader) public javax.script.ScriptEngineManager() javax.script.ScriptEngineManager@44eb5bdd jdk.nashorn.api.scripting.NashornScriptEngine@6b81dee4 [Ljava.lang.reflect.Method;@1c26a009 public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.lang.String) throws javax.script.ScriptException public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.io.Reader) throws javax.script.ScriptException public javax.script.ScriptEngineFactory jdk.nashorn.api.scripting.NashornScriptEngine.getFactory() public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.lang.String,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.io.Reader,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Object,java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeMethod(java.lang.Object,java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeFunction(java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public javax.script.Bindings jdk.nashorn.api.scripting.NashornScriptEngine.createBindings() public java.lang.Object javax.script.AbstractScriptEngine.get(java.lang.String) public void javax.script.AbstractScriptEngine.put(java.lang.String,java.lang.Object) public javax.script.ScriptContext javax.script.AbstractScriptEngine.getContext() public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String,javax.script.Bindings) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader,javax.script.Bindings) throws javax.script.ScriptException public void javax.script.AbstractScriptEngine.setContext(javax.script.ScriptContext) public javax.script.Bindings javax.script.AbstractScriptEngine.getBindings(int) public void javax.script.AbstractScriptEngine.setBindings(javax.script.Bindings,int) public final void java.lang.Object.wait() throws java.lang.InterruptedException public final void java.lang.Object.wait(long,int) throws java.lang.InterruptedException public final native void java.lang.Object.wait(long) throws java.lang.InterruptedException public boolean java.lang.Object.equals(java.lang.Object) public java.lang.String java.lang.Object.toString() public native int java.lang.Object.hashCode() public final native java.lang.Class java.lang.Object.getClass() public final native void java.lang.Object.notify() public final native void java.lang.Object.notifyAll() $f.eval("var B=Java.type('java.util.Base64');var F=Java.type('java.io.FileOutputStream');var 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Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (11): 1633-1636.

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Effect of calcineurin Aα gene overexpression on the myocardium apoptosis induced by hypoxia-reoxygenation and adrenergic receptors

SHEN Xiao-mei1,2,ZHANG Ju-yan2,CHENG Bei1 1Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;2First Affiliated Hospital,Shanxi Medical University,Taiyuan 030001,China   

  1. 华中科技大学同济医学院协和医院心血管内科； 山西医科大学第一附属医院； 华中科技大学同济医学院协和医院心血管内科 湖北武汉430022山西医科大学第一附属医院； 山西太原030001； 湖北武汉430022；
  • Online:2006-11-20 Published:2006-11-20

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Abstract: Objective To investigate the effect of calcineurin AαcDNA(AdCnAα) overexpression as a result of adenovirally mediated gene transfer on neonatal rat cardiac myocyte apoptosis induced by hypoxia-reoxygenation(H/R) and adrenergic receptors.Methods Neonatal rat cardiac myocytes were cultured for 20 h after AdCnAα transfection,and treated with isoproterenol(10 μmol/L) and 24 h of hypoxia followed by 4 h of reoxygenation(24H/4R).The cardiac myocyte apoptosis induced by the treatments was assessed by flow cytometry and DNA laddering,and the levels of calcineurin,p38 and phosphorylation p38(p-p38) were determined by Western blotting and(or) RT-PCR.Results AdCnAα transfection promoted cultured neonatal rat cardiac myocyte apoptosis induced by isoproterenol+24H/4R as compared with the treated cells without transfection(14.247±0.525 vs 10.763±1.554,P<0.01),along with greater phosphorylation p38 protein expression(1.60±0.22 vs 2.42±0.19,P<0.01).The levels of p38 underwent no obvious change after AdCnAα transfection(P<0.05).Conclusions AdCnAα transfection can promote cardiac myocyte apoptosis induced by H/R and adrenergic receptors,the mechanism of which might be associated with p38 mitongen-activated protein kinase(p38MAPK) activation. 

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