Journal of Southern Medical University

Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (10): 1460-1463.

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ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei)

XIE Hui1, CHAO Zhi2, HUO Ke-ke3, WU Bing-yi4, PAN Sheng-li1 1School of Pharmacy, Fudan University, Shanghai 200032, China; 2School of Tradition Chinese Medicine, Southern Medical University, Guangzhou 510515, China; 3Institute of Biological Science, Fudan University, Shanghai 200433, China; 4Clinical Research Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 复旦大学药学院生药教研室； 南方医科大学中药学院； 复旦大学生命科学研究院； 南方医科大学南方医院临床实验研究中心； 复旦大学药学院生药教研室 上海200321； 广东广州510515； 上海； 200433； 上海200321；
  • Online:2006-10-20 Published:2006-10-20

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Abstract: Objective The study the patterns of rDNA ITS sequence variation of 9 medicinal species of genus Bupleurum in China to find the DNA molecular markers for identification of these crude drugs. Method The ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DNAssist Version 2.2. Results Homologous alignment indicated that the consanguinity of the ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. For the same species, the consanguinity was higher than 99%. Conclusion The ITS sequences may serve as reliable molecular markers for identification of Radix Bupleuri. 

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