Sequence analysis of the mutated genes in CTXEVCφ and nct-CTXnewφ genomes in Vibrio cholerae JS94484 strain

Abstract

Abstract: Objective To analyze the sequences of the mutated genes in CTX^EVC_φ and nct-CTX^new_φ genomes in Vibrio cholerae JS94484 strain. Methods The mutated genes in CTX^EVC_φ and nct-CTX^new_φ genome were obtained by PCR, sequenced and analyzed. Results ig1, rstR, ig2, and ctxAB genes in CTX^EVC_φ genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTX^new_φ genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTX^EVC_φ and nct-CTX^new_φ genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961. Conclusion ig1, rstR and ig2 genes of nct-CTX^new_φ genome are of a novel type, and their functions await further investigation.

CLC Number:

R379

RUI Yong-yu¹, KAN Biao², GAO Shou-yi², LIU Yan-qing², QI Guo-ming². Sequence analysis of the mutated genes in CTX^EVC_φ and nct-CTX^new_φ genomes in Vibrio cholerae JS94484 strain[J]. Journal of Southern Medical University, 2005, 25(11): 1361-1364.

References

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Sequence analysis of the mutated genes in CTX^EVC_φ and nct-CTX^new_φ genomes in Vibrio cholerae JS94484 strain

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