Differentiation of neonatal rat striatal neural stem cells induced by all-trans retinoic acid

Abstract

Abstract: Objective To investigate the role of all-trans retinoic acid (atRA) in inducing differentiation of neonatal rat striatal neural stem cells (NSCs). Methods Neonatal rat striatal NSCs were obtained by mechanical isolation and serum-free culture. The roles of atRA at different concentrations in inducing the differentiation of NSCs were observed by immunofluorescent cytochemical staining, and the expression of retinoic acid receptor (RAR) gene was determined by semi-quantitative RT-PCR. Results atRA could dose-dependently accelerate the differentiation of NSCs into neuron-like cells, and the physiological concentration of atRA was optimal for inducing NSC differentiation. atRA could induce the expression of RARβ mRNA in a dose- and time-dependent manner. Conclusion atRA can accelerate differentiation of NSCs into neuron-likes cells and up-regulate the expression of RARβ mRNA in neonatal rat striatal NSCs.

CLC Number:

R-33

DENG Hong, ZOU Fei, LUO Hai-ji. Differentiation of neonatal rat striatal neural stem cells induced by all-trans retinoic acid[J]. Journal of Southern Medical University, 2005, 25(11): 1357-1360,1374.

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