Dynamic monitoring of graft-versus-host disease after allogeneic peripheral blood stem cell transplantation by soluble HLA-I antigen detection

Abstract

Abstract: Objective To assess the value of dynamic monitoring of soluble human lymphocytic antigen-I (sHLA-I) in the prediction of graft-versus-host disease (GVHD) after allogeneic peripheral blood stem cell transplantation (PBSCT). Method Sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantitatively detect serum sHLA-I. The serum samples for testing were added into W6 32 monoclonal antibody-coated microtiter plate and incubated with anti-β2m HRP followed by color development with the addition of the substrate. Serum sHLA-I level was measured in 63 healthy blood donors of Shanghai and in 24 PBSCT recipients before and and at different time points after the operation. Result No changes in sHLA-I levels occurred in allogeneic PBSCT recipients without GVHD or with only gradeⅠGVHD, but sHLA-I reached high levels in patients suffering GVHDⅡ-Ⅳ(P<0.05), which was effectively lowered by the application of immunosuppressants. Conclusion Measurements of sHLA-I levels can be valuable for monitoring GVHD after PBSCT.

CLC Number:

R329.24

XIA Rong^1,2, ¹, QIU Hui-ying¹, ZHANG Wei-ping², CAO Qiong², LAN Jiong-cai¹, WEN Hong-sheng¹. Dynamic monitoring of graft-versus-host disease after allogeneic peripheral blood stem cell transplantation by soluble HLA-I antigen detection[J]. Journal of Southern Medical University, 2005, 25(06): 687-690,695.

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