Effects of macrophage migration inhibitory factor on collagenⅠ and Ⅲ mRNA expression in vascular smooth muscle cells in vitro

Abstract

Abstract: Objective To investigate the association between macrophage migration inhibitory factor (MIF) and collagen synthesis in vascular smooth muscle cells (VSMCs). Method VSMCs isolated from SD rat aorta were cultured in different culture plates, and the fourth passage of cells were divided into two groups, one of which was simulated by MIF and the other served as control group. CollagenⅠand Ⅲ mRNA expression in the VSMCs was detected by reverse transcriptase (RT)-PCR and PCR. Result CollagenⅠand Ⅲ mRNA expression in MIF-treated VSMCs increased significantly as compared with that in the control cells (P<0.05). Conclusion MIF increases collagenⅠand Ⅲ mRNA expression of VSMCs in vitro, indicating its involvement in collagen synthesis in VSMCs.

CLC Number:

R329.2

LI Jun, LIN Shu-guang, YU Xi-yong, LIN Qiu-xiong. Effects of macrophage migration inhibitory factor on collagenⅠ and Ⅲ mRNA expression in vascular smooth muscle cells in vitro[J]. Journal of Southern Medical University, 2005, 25(06): 684-686.

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