Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis

Abstract

Abstract: Objective To evaluate the specificity and sensitivity of immunodiagnosis with the antigen with molecular mass of 32 000 of Angiostrongyliasis cantonensis(AC32). Methods The major antigenic protein AC32 was purified from the antigens of A.cantonensis adult worm by electroelution from SDS-polyacrylamide gels. AC32-enzyme linked immunosorbent assay (ELISA) and adult worm antigen (AWA)-ELISA were used to detect the specific IgG in the sera of normal rats (n=5) and rats with angiostrongyliasis (n=61), sera of healthy individuals (n=50) and patients (n=50) with angiostrongyliasis, schistosomiasis and otherparasitosis. Results The positivity rate in AC32-ELISA of the sera from rats with angiostrongyliasis was 100% without false positive results or cross reaction between AC32 and the sera of the normal control and patients infected with parasites other than A.cantonensis. Conclusion AC32 of A.cantonensis is a valuable candidate antigen for immunodiagnosis of angiostrongyliasis with high sensitivity and specificity.

CLC Number:

LI Hua¹, CHEN Xiao-guang¹, SHEN Hao-xian², CHEN Dai-xiong², QIU Yu-rong³, HU Xiao-jia³. Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis[J]. Journal of Southern Medical University, 2005, 25(04): 380-383.

References

[1] 梁浩昆.关于广州管园线虫的概述[J].广州医学院学报(ActaGuangzhou Med Coll),1988,16(1):95-101.
[2] 潘长旺,凌洪博,梁韶晖,等.ELISA检测广州管圆线虫感染实验大鼠血清抗体[J].中国人兽共患病杂志,2000,16(1):79-80.Pan CW, Ling HB, Liang SH, et al. Detection of antibodies in rats infected with Angiostrongylus cantonensis by ELISA[J]. Chin J Zoon, 2000, 16(1): 79-80.
[3] 李华,陈晓光,沈浩贤,等.不同发育阶段广州管园线虫抗原分析[J].中国寄生虫学与寄生虫病杂志,2005,23(1):2033-6.Li H, Chen XG, Shen HX, et al. Antigen analysis of Angiostrongylus cantonensis in different development stages[J]. Chin J Parasitol Parasitic Dis, 2005, 23(1): 2033-6.
[4] 李华,汪世平,曾宪芳,等日本血吸虫未成熟虫卵67 kDa分子抗原诊断血吸虫病的研究[J].中国血吸虫病防治杂志,1998,(5):271-4.Li H, Wang SP, Zeng XF, et al. Immnodiagnosis of Schitosomiasis japonica using 67 kDa antigenic molecule purified from soluble immature egg antigen[J]. Chin J Schistosomiasis Control, 1998,(5): 271-4.
[5] 李华,易新元,曾宪芳,等.日本血吸虫成虫67 kDa分子抗原的纯化及其对血吸虫病的诊断和疗效考核[J].中国热带医学,2001,1(2):93-5.Li H, Yi XY, Zeng XF, et al. The purification of 67 kDa antigen from AWA of Schistosomajaponica and its application in diagnosis and assessment of curative effect[J] Chin Trop Med, 2001, 1 (2):93-5.
[6] Maleewong W, Sombatsawat P, Intapan PM, et al. Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms A ngiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis[J]. Asian Pac J Allergy Immunol, 2001,19(4): 267-73.
[7] Chye SM, Chang JH, Yen CM. Immunodiagnosis of human eosinophilic meningitis using an antigen ofAngiostrongylus cantonensis L5with molecular weight 204 kD[J]. Acta Trop, 2000, 75 (1): 9-17.
[8] Eamsobhana P, Yoolek A, Suvouttho S, et al. Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels[J]. Southeast Asian J Trop Med Public Health, 2001, 32(2): 308-13.
[9] Wongkham C, Maleewong W, Intapan P, et al. Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis[J]. Southeast Asian J Trop Med Public Health,1994, 25(1): 176-80.
[10] Nuamtanong S. The evaluation of the 29 and 31 kDa antigens in female Angiostrongylus cantonensis for serodiagnosis of human angiostrongyliasis[J]. Southeast Asian J Trop Med Public Health,1996, 27(2): 291-6.
[11] 黄绪强,钟传昌,何竟智.免疫酶染色试验检测广州管园线虫感染鼠血清抗体的敏感性和特异性[J].中国人兽共患病杂志(Chin J Zoon),1994,10(1):28-9.
[12] 王小同,李方去,黄汉津,等.酶联免疫吸附试验测定广州管园线虫病患者血清抗体的临床意义[J].中国神经免疫学和神经病学杂志,1999,6(2):128-30.Wang XT, Li FQ, Huang HJ, et al. Clinical significance of the measurement of sera antibody angaint Angiostrongylus cantonensis by enzyme-linked immunosorbent assay[J]. Chin J Neuroimunol Neurol, 1999, 6(2):128-30.