Association of interleukin-6 induction and the novel gene LX3

Abstract

Abstract: Objective To analyze the association between the expression of the novel gene LX3 and interleukin-6 (IL-6) induction, and explore a new target gene for action mechanism of IL-6. Methods The total RNA was extracted from U937 cells induced by IL-6 at different concentrations and varied lengths of time. Reverse transcriptional (RT)-PCR and Northern blotting were employed to determine the expression of LX3 and IL-6 induction. Results The expression of novel gene LX3 was increased while concentration of IL-6 was improved gradually. High expression was induced by IL-6, which was highest at the concentration was 500 ng/ml and no expression at 0 ng/ml. Time- expression pattern proved that expression of novel gene LX3 of higest at 8 hour after induced by IL-6. Northern blotting confirmed that the expression quantity of LX3 increased in U937 cells induced by IL-6. Conclusion The quantity of LX3 expression is associated with the dose and duration of IL-6 induction, suggesting that LX3 gene is a novel gene correlated with IL-6 induction.

CLC Number:

XIA Rong^1,2, LI Yan², FENG Jian-nan², LAN Jiong-cai¹, SHEN Bei-fen². Association of interleukin-6 induction and the novel gene LX3[J]. Journal of Southern Medical University, 2005, 25(04): 384-386.

References

[1] Xia Q, Wang HX, Wang J. Proteomic analysis of interleukin 6-induced differentiation in mouse myeloid leukemia cells[J]. Int J Biochem Cell Biol, 2005, 37(6): 1197-207.
[2] Fischer P, Lehmann U, Sobota RM. The role of the inhibitors of interleukin-6 signal transduction SHP2 and SOCS3 for desensitization of interleukin-6 signalling[J]. Biochem J, 2004, 378(Pt 2): 449-60.
[3] Hollestelle A, Schutte M. Representational difference analysis as a tool in the search for new tumor suppressor genes[J]. Methods Mol Med, 2004, 103: 143-60.
[4] Ramalho-Ortigao JM, Temporal P, Oliveira SM. Characterization of constitutive and putative differentially expressed mRNAs by means of expressed sequence tags, differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR from the sand fly vector Lutzomyia longipalpis[J]. Mem Inst Oswaldo Cruz, 2001, 96(1): 105-11.
[5] 夏荣,黎燕,沈倍奋,等IL-6相关新基因的生物学分析[J].第一军医大学学报,2003,23(1):34-7.Xia R, Li Y, Shen BF, et al. Biological analysis of a novel related gene with interleukin-6[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2003, 23(1): 34-7.
[6] Tang G, Samaranayake LP, Yip HK. Direct detection of Actinomyces spp from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide-DNA hybridization technique[J].J Dent, 2003, 31(8): 559-68.
[7] Cross JT, Benton HP. The roles of interleukin-6 and interleukin-10in B cell hyperactivity in systemic lupus erythematosus[J]. Inflamm Res, 1999, 48(5): 255-61.
[8] 黄耀江,黎燕,沈倍奋,等用一个简单快速的RT-PCR技术筛选白细胞介素-6作用相关基因[J].生物化学与生物物理,2001,28(4):563-8.Huang YJ, Li Y, Sheng BF, et al. Isolation of genes related with IL-6effect by a rapid and simple RT-PCR method[J]. Prog Biochem Biophy, 2001, 28(4): 563-8.

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