Expression of multidrug resistance gene in nasopharyngeal carcinoma cells after irradiation exposure

Abstract

Abstract: Objective To examine the changes in the function and expression of multidrug resistance gene (mdr1) and P-gly-coprotein (P-gp) in nasopharyngeal carcinoma (NPC) CNE1 cell following irradiation for determining the sequential order of radiotherapy and chemotherapy in the treatment of NPC. Methods The expressions of mdr1 gene and its protein P-gp as well as the function of P-gp efflux were examined in CNE1 cells before and after irradiation exposure by reverse transcriptional polymerase chain reaction (RT-PCR), Western blotting and flow cytometry, respectively. Results Irradiation of CNE1 cells induced a long-term overexpression of mdr1 gene and P-gp and reduction in intracellular daunorubicin accumulation. Conclusion Irradiation decreases the chemotherapy sensitivity of CNE1 cell, and induction chemotherapy should be therefore performed before radiotherapy in the treatment of advanced NPC.

CLC Number:

R394.7:R739.63

BU Jun-guo, YUAN Ya-wei, CHEN Jun. Expression of multidrug resistance gene in nasopharyngeal carcinoma cells after irradiation exposure[J]. Journal of Southern Medical University, 2004, 24(06): 646-649.

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