UrotensinⅡinduces hypertrophy of in vitro cultured neonatal rat cardiac myocytes

Abstract

Abstract: Objective To examine whether urotensinⅡ(UⅡ) induces hypertrophy of neonatal rat cardiomyocytes cultured in vitro. Methods The primary cardiac myocytes cultured for 40 h followed by further culture in serum-free media for another 24 h were subjected to exposure to UⅡof varied concentrations for 24 h, after which the changes in the size of the cells were analyzed by flow cytometry with ³H-leucine incorporation also measured. Results At the concentration of 1×10^-7 mol/L, UⅡcould increase the size of the cultured cardiac myocardial cells (P=0.021) and ³H-leucine incorporation (P=0.015). Conclusion UⅡmay induce hypertrophy of neonatal rat cardiac myocytes cultured in vitro.

CLC Number:

ZUO Hui-hua¹, HONG Jie-min², GUO Heng-shan¹, WEI Jian-rui¹, HE Yan-hua¹, LI Zhi-liang³. UrotensinⅡinduces hypertrophy of in vitro cultured neonatal rat cardiac myocytes[J]. Journal of Southern Medical University, 2004, 24(06): 642-645.

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