Cloning and sequence analysis of the preprotein cDNA of human bone morphognetic protein 12

Abstract

Abstract: Objective To clone the cDNA of the preprotein cDNA of human bone morphogenetic protein 12 (hBMP12). Methods Two primers were designed according to hBMP12 sequence reported in GenBank. The hBMP12 preprotein cDNA was obtained by reverse transcriptional (RT)-PCR from the mRNA extracted from human placenta, followed by cloning into pT_ARGET^TM plasmid and sequence analysis of the plasmid pTARGETTM-BMP12. Results DNA agarose gel electrophoresis showed that the product of RT-PCR was about 920 bp, as was consistent with the result of PCR detection of the recombinant plasmid. The result of sequence analysis was in agreement with the reported hBMP12 sequence in GenBank. Conclusion The preprotein of hBMP12 cDNA has been successfully cloned with correct sequence.

CLC Number:

LU Yan-meng¹, FU Wen-yu², PIAO Ying-jie¹. Cloning and sequence analysis of the preprotein cDNA of human bone morphognetic protein 12[J]. Journal of Southern Medical University, 2004, 24(03): 260-263.

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