南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (5): 749-754.doi: 10.12122/j.issn.1673-4254.2023.05.10

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环状RNA hsa_circ_0087893可能通过ceRNA机制参与早产儿脑室内出血的发生和发展

陈茹娟，武 伟，邱银萍   

  1. 宁夏医科大学总医院新生儿科，宁夏 银川 750000
  • 出版日期:2023-05-20 发布日期:2023-06-12

Circular RNA hsa_circ_0087893 participates in intraventricular hemorrhage occurrence and progression possibly as a competitive endogenous RNA in preterm infants

CHEN Rujuan, WU Wei, QIU Yinping   

  1. Department of Neonatology, General Hospital of Ningxia Medical University, Yinchuan 750000, China
  • Online:2023-05-20 Published:2023-06-12

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摘要： 目的 筛选发生脑室内出血（IVH）早产儿血清中差异表达的环状RNA（circRNA），并探讨circRNA参与早产儿IVH发生、发展的ceRNA机制。方法 研究对象为2019年1月~2020年1月在我院新生儿科住院，胎龄28~34周的早产儿共50例。采用circRNA芯片技术筛选在IVH早产儿血清中显著差异表达的circRNA，通过基因本体论（GO）和信号通路分析circRNA的功能，荧光定量PCR验证hsa_circ_0087893在两组的实际表达水平，构建hsa_circ_0087893的circRNA-miRNA-mRNA共表达网络。结果 共有121条circRNA在早产IVH组显著差异表达，包括62条上调表达circRNA及59条下调表达circRNA。GO分析及信号通路分析表明，差异circRNA参与多种生物功能及信号通路，包括细胞增殖、活化及死亡、DNA损伤及修复、维生素A代谢、鞘磷脂代谢及细胞黏附等。hsa_circ_0087893在早产儿IVH组中表达显著下调（P<0.05），41个miRNA与hsa_circ_0087893共表达，包括 miR-214-3p、miR-761、miR-183-5p 等。15 条 mRNA 与 hsa_circ_0087893 共表达，包括 AKR1B1、KRT34、PPP2CB、HPRT1等。结论 hsa_circ_0087893可能通过ceRNA机制调控早产儿IVH发生、发展。

关键词: 早产儿, 脑室内出血, 芯片, 竞争性内源性RNA, 机制

Abstract: Objective To screen for differentially expressed circular RNAs (circRNAs) in the serum of preterm infants with intraventricular hemorrhage (IVH) and explore the competitive endogenous RNA (ceRNA) mechanism of circRNAs in IVH in these infants. Methods Fifty preterm infants (gestational age of 28 to 34 weeks) admitted in our department between January, 2019 and January, 2020 were enrolled in this study, including 25 with a MRI diagnosis of IVH and 25 without IVH. Serum samples were collected from 3 randomly selected infants from each group for profiling differentially expressed circRNAs using circRNA array technique. Gene ontology (GO) and pathway analyses were performed to reveal the function of the identified circRNAs. The circRNA-miRNA-mRNA network was constructed to identify the co-expression network of hsa_circ_0087893. Results A total of 121 differentially expressed circRNAs were identified in the infants with IVH, including 62 up-regulated and 59 down-regulated circRNAs. GO and pathway analyses showed that these circRNAs were involved in multiple biological processes and pathways, including cell proliferation, activation and death, DNA damage and repair, retinol metabolism, sphingolipid metabolism, cell adhesion molecules. Among these circRNAs, hsa_circ_0087893 was found to have significant down-regulation in IVH group and co-express with 41 miRNAs and 15 mRNAs (such as miR-214-3p, miR-761, miR-183-5p, AKR1B1, KRT34, PPP2CB, and HPRT1). Conclusion The circRNA hsa_circ_0087893 may function as a ceRNA and play an important role in the occurrence and progression of IVH in preterm infants.

Key words: preterm infants, intraventricular hemorrhage, microarray, competitive endogenous RNA, mechanism