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Mental health status and its influencing factors among college students during the epidemic of COVID-19 Journal of Southern Medical University    2020, 40 (02): 171-176.   DOI: 10.12122/j.issn.1673-4254.2020.02.02 Abstract （24358）      PDF（pc） （727KB）（6281）       Knowledge map    Save Objective To investigate the mental health status of college students during the epidemic of COVID-19 and identify the factors influencing the mental health of the students. Methods Using a general questionnaire, a self-designed new coronavirus pneumonia knowledge and cognitive behavior questionnaire, the Generalized Anxiety Disorder 7 (GAD-7) and Patient Health Questionnaire 9 (PHQ-9), we conducted an internet-based questionnaire survey of 3881 college students in Guangdong Province. A multinomial-logistic regression model was used to analyze the collected data. Results The survey showed that 69.47% of the college students had a high level of awareness of COVID-19; the overall incidence of anxiety was 26.60% , and the incidences of mild, moderate and severe anxiety were 23.19% , 2.71% , and 0.70% , respectively. Depressive emotions were detected in 21.16% of the students, and the incidences of mild, moderate, and moderate-to-severe depression were 16.98%, 3.17%, and 1.01%, respectively. The results of multivariate analysis showed that an older age was associated with a higher level of awareness of COVID-19 and greater changes in future health behaviors were associated with less anxiety and depression among the students. The students currently in rural areas, of non-medical majors, and reporting half of their information concerning the epidemic being negative were more likely to have anxiety; female gender, residence in suburbs, a drinking history, and excessive negative information concerning the epidemic were all associated with the likeliness of depression. Conclusion The college students have different levels of anxiety and depression during the epidemic. Depression and anxiety are closely related, but the factors contributing to different levels of such emotions can be different, and colleges and related departments are urged to provide precision mental health education for college students. Reference | Related Articles | Metrics | Comments（0）

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Dandelion clock-like sign on CT for diagnose of COVID-19 Journal of Southern Medical University    2020, 40 (02): 159-163.   DOI: 10.12122/j.issn.1673-4254.2020.02.06 Abstract （8331）      PDF（pc） （1844KB）（5061）       Knowledge map    Save Objective To explore the value of the "dandelion clock-like" sign on chest CT for diagnosis of SARS-CoV-2-associated pneumonia. Methods This retrospective analysis was conducted based on the data of 119 cases from the Department of Fever and the Department of Infection undergoing chest high-resolution CT examinations in Sanshui District People’s Hospital between January, 24 and February 10, 2020. The cases with no abnormality on chest CT were excluded. Twenty-three patients were diagnosed to have pneumonia, including 9 with SARS-CoV-2-associated pneumonia and 14 with other types of pneumonia. We comparatively analyzed the CT signs, location of the lesions and the dandelion clock-like sign among different types of pneumonia. Results Among the 23 patients with pneumonia, 9 (39.1% ) had common or severe SARS-CoV-2- associated pneumonia with a positive epidemiological history and corresponding respiratory symptoms. Seven of the SARSCoV-2-associated pneumonia patients had multiple lesions in bilateral lungs, compromising mainly the lung field and the subpleural area and showing patchy, lumpy, and umbrella-shaped ground glass opacity, often accompanied by pulmonary vascular thickening and increased microvessels, interlobular septal thickening and fibrosis and lined with grid-like and small-bubble-like "crazy-paving" patterns. The dandelion clock-like sign was found in all the 9 patients with SARSCoV-2-associated pneumonia, with a total of 46 lesions (60.5% , 76 total lesions); 9 of the lesions presented with a "dandelion clek-like" sign and 37 with a "dandelion seed sign". These signs were not found in the 14 patients with other types of pneumonia. Conclusion The dandelion clock-like sign is a common and characteristic chest CT finding in patients with SARS-CoV-2-associated pneumonia, and can help to distinguish SARS-CoV-2-associated pneumonia from other types of pneumonia. Reference | Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (07): 1220-.   Abstract （7048）      PDF（pc） （1288KB）（7060）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Effects of sera of rats fed with Huganqingzhi tablets on endoplasmic reticulum stress in a HepG2 cell model of nonalcoholic fatty liver disease Journal of Southern Medical University    2018, 38 (11): 1277-.   DOI: 10.12122/j.issn.1673-4254.2018.11.01 Abstract （6725）      PDF（pc） （1232KB）（5622）       Knowledge map    Save Objective To investigate the effects of sera from rats fed with Huganqingzhi tablets (HGT) on endoplasmic reticulum (ER) stress in a steatotic hepatocyte model of free fatty acids (FFAs)- induced nonalcoholic fatty liver disease (NAFLD) and explore the possible mechanism. Methods FFAs prepared by mixing oleic acid and palmitic acid at the ratio of 2∶1. HepG2 cells were treated with the sera from rats fed with low- , moderate-or high-dose HGT (HGT sera) or sera of rats fed with fenofibrate (fenofibrate sera), followed by treatment with 1 mmol/L FFAs for 24 h to induce hepatic steatosis. Oil red O staining was used to observe the distribution of lipid droplets in the cells. The biochemical parameters including triglyceride (TG), lactated hydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured using a commercial kit. The morphological changes of the ER in the cells were observed using transmission electron microscopy. The protein/mRNA expressions of ER stress-related signal molecules including GRP78, PERK, p-PERK, ATF6, ATF4, CASPASE-12, CHOP, XBP-1, PKC, and p-PKC-δ were detected using Western blotting and/or quantitative real- time PCR (qRT- PCR). The changes in the protein expressions of GRP78, p-PERK, CASPASE-12 and CHOP were also detected in cells with transient transfection of PKC-δ siRNA for PKC-δ knockdown. Results Compared with the control cells, the cells treated with FFAs showed significantly increased levels of TG, AST, and ALT (P<0.05). Compared with FFAs- treated cells, the cells pretreated with HGT sera or fenofibrate sera all showed significantly decreased TG, AST and ALT levels (P<0.05), reduced accumulation of the lipid droplets (P<0.05), and lowered protein or mRNA expression levels of GRP78, p-PERK, ATF6, ATF4, CHOP, CASPASE-12, XBP-1 and p-PKC-δ (P< 0.05). PKC-δ knockdown caused significantly reduced protein expressions of GRP78, p-PERK, CASPASE-12 and CHOP in the cells with FFA- induced hepatic steatosis (P<0.001); treatment with high-dose HGT serum more significantly reduced the expressions of GRP78 (P<0.001) and P-PERK (P<0.01) in FFAs-induced cells with PKC-δ knockdown. Conclusion HGT serum can effectively prevent FFAs-induced steatosis in HepG2 cells by alleviating ER stress, in which PKC-δ may act as an important target. Related Articles | Metrics | Comments（0）

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Comparative investigation of the Rh blood type distribution between the Uygur and Han nationalities in the Khotan area of Xinjiang Autonomous Region Kurexijiang Tuohuti1, Hamulati Wufuer2, Nuermaimaiti Yili1, Muyasaier Kurexi1, Palida Habaer Ruzi1, Halike Yimi-ti1, Meilike Yihepa1, ZHANG Li-zhan1, Maimaitiabula Aisha1 Journal of Southern Medical University    2004, 24 (04): 447-449.   Abstract （6385）      PDF（pc） （171KB）（4297）       Knowledge map    Save Objective To investigate the Rh blood type distribution in the Uygur and Han nationalities in Khotan area of Xin-jiang Autonomous Region, China, and compare the results with previous documentations on the Rh blood type in Uighurs. Method Using epidemiological methods, an extensive survey was conducted for determination of the Rh blood type in 2 907 residents in the target area, including 2 251 Uighurs and 656 subjects of Han nationality. Positive definition method was used for the ABO blood typing while Rh blood type was determined serologically through saline medium method. At the same time, the Rh phenotypes were investigated in RhD-negative individuals. Results Altogether 106 RhD-negative individuals were identified, accounting for a rate of 4.71% in this cohort, with the D gene frequency of 0.217. The Rh phenotype of all RhD- negative cases were ccdee except for one that was ccdEe. When compared with the previous ABO blood type distribution data of the Uighurs in randomly chosen samples, B type in RhD-negative individuals was relatively higher while A and O types peared lower. Conclusion The Rh blood type frequency is relatively higher in the Uighurs with unique Rh phenotypes. Reference | Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (11): 1900-.   Abstract （6345）      PDF（pc） （1270KB）（5074）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (04): 572-.   Abstract （6261）      PDF（pc） （1364KB）（7804）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2012, 32 (04): 467-.   Abstract （6229）      PDF（pc） （2120KB）（3988）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Jiangtang Sanhuang tablet inhibits endoplasmic reticulum stress and autophagy in diabetic mouse islet cells ZHANG Wenjing, HU Zhaoting Journal of Southern Medical University    2022, 42 (9): 1317-1323.   DOI: 10.12122/j.issn.1673-4254.2022.09.07 Abstract （5977）   HTML （45）    PDF（pc） （1911KB）（425）       Knowledge map    Save Objective To investigate effects of Jiangtang Sanhuang tablet (JTSHT) for regulating blood glucose and alleviating islet cell damage in db/db mice and its protective effects against endoplasmic reticulum stress (ERS) and autophagy induced by glycolipid toxicity. Methods Forty db/db mice were randomized into 4 groups for daily intragastric administration of saline, JTSHT of 2.64 and 1.32 g/kg, and metformin at 0.225g/kg for 8 weeks, using 10 C57BL/6J mice as the normal control. After the treatments, the metabolic indexes of the mice were measured, and morphological changes of the islet cells were observed. A mouse islet cell line (MIN6) was exposed to high glucose (22 mmol/L glucose) and 0.1 mmol/L palmitic acid, followed by treatment with the sera from JTSHT- or saline- treated SD rats, alone or in combination with SP600125, and the changes in cell apoptosis, ERS and autophagy were evaluated using flow cytometry, RT-qPCR and Western blotting. Results In db/db mice, treatment with JTSHT significantly improved glucose and lipid metabolism (P<0.05) and suppressed progressive weight gain (P<0.05) without significant effect on drinking water volume (P>0.05). JTSHT was also found to promote repair of islet cell injuries. In the cell experiments, high glucose exposure significantly increased apoptosis rate of MIN6 cells (P<0.05), which was obviously lowered by treatment with JTSHT-treated rat serum (P<0.05). Western blotting showed that JTSHT significantly reduced the level of ERS and autophagy caused by glycolipid toxicity in MIN6 cells (P<0.05). Interference with ERS using SP600125 significantly attenuated the protective effect of JTSHT against MIN6 cell injury, apoptosis and autophagy induced by glycolipid toxicity (P<0.05). Conclusion JTSHT has protective effects against glycolipid toxicity in MIN6 cells possibly by inhibiting ERS and autophagy. Related Articles | Metrics | Comments（0）

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Difference in HLA-A*02 allele distribution between Han populations in south and north China CHENG Liang-hong, JIN Shi-zheng, GAO Su-qing, LI Zhen, ZOU Hong-yan, WANG Da-ming, WU Guo-guang Journal of Southern Medical University    2005, 25 (03): 321-324.   Abstract （5950）      PDF（pc） （781KB）（3442）       Knowledge map    Save Objective To investigate the distributions of HLA-A∗02 alleles in Han populations and compare their difference between the south and north in China.Methods A total of 208 individuals from south China and 109 from north China were randomly selected from registered bone marrow donors in Chinese Han population, who were tested positive for HLA-A*02 alleles by PCR with sequence-specific primers (PCR-SSP). Genotyping of the alleles was performed using PCR-sequence-based typing (PCR-SBT).Results Six different HLA-A∗02 alleles (A∗020101, A∗0203, A∗0205, A∗0206, A∗0207 and A∗0210) were identified in the two Chinese Han populations, of which A∗0207(37%) was the predominant allele in southerners and A∗020101(48%) in the northerners. A∗020101(31%), A∗0203(16%) and A∗0206(14%) were common alleles in the southerners in comparison with ∗#8727;0206(21%) and A∗0207(23%) alleles in the northerners. The overall distribution of A∗02 alleles and the frequencies of A∗020101, A∗0203 and A∗0207 in the two populations differed significantly. The heterozygosity of A∗02 in the southerners and northerners was higher than 90% and 80% at the high- and low-resolution levels, respectively, and the distribution of A∗02 homozygote at low-resolution level in both populations showed diversity and regularity at high-resolution level.Conclusions HLA-A∗02 alleles possess high heterogeneity and genetic diversity in Chinese Han population with significantly different distributions in the two populations. HLA-A∗020101, A∗0203 and A∗0207 may serve as the genetic markers for dividing Chinese Han individuals into southerners and northerners in anthropological studies. Reference | Related Articles | Metrics | Comments（0）

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PKD3 contributes to up-regulation of prostate-specific antigen in prostate cancer cells  DENG Fan, WANG Chun-xia, XU Wan-fu, FENG Li, KE Zhi-yong, Wang Q. Jane, ZOU Zhi-peng Journal of Southern Medical University    2010, 30 (08): 1779-1782.   Abstract （5662）      PDF（pc） （1166KB）（6077）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Study of three-dimensional speech chart by time-frequency analysis in Chinese Mandarin Monosyllabic word phonemes  CHEN Hao, QIAN Yu-hong, ZHOU Xiao-qin, GUO Meng-he Journal of Southern Medical University    2010, 30 (08): 1805-1809.   Abstract （5499）      PDF（pc） （2679KB）（4795）       Knowledge map    Save Reference | Related Articles | Metrics | Comments（0）

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Construction of prokaryotic expression plasmid pET15b-PEP-1-CAT and expression and purification of PEP-1-CAT fusion protein YAO Ling-ling, WANG Jia-ning, HUANG Yong-zhang, GUO Ling-yun Institute of Clinical Medicine, People’s Hospital, Yunyang Medical College, Shiyan 442000, China Journal of Southern Medical University    2006, 26 (09): 1319-1325.   Abstract （5359）      PDF（pc） （860KB）（3416）       Knowledge map    Save Objective To construct the prokaryotic expression plasmid pET15b-PEP-1-CAT to obtain purified fusion protein of PEP-1-CAT. Methods Using pfu DNA polymerase, the full-length human catalase cDNA was amplified by PCR from pZeoSV2(+)-CAT plasmid, and the PCR product was added with "A" using Taq DNA polymerase. The purified product of CAT cDNA with the base A at its 3’ end was ligated with pGEM-T Easy vector and transformed into DH5α. The correct recombinant was identified by PCR and SalⅠ/BglⅡ digestion and named as pGEM-T-CAT. Two oligoncleotides were synthesized and annealed to generate a double-stranded oligonucleotide encoding the PEP-1 peptide, which was directly ligated into Nde I/Xho I-digested pET15b.The recombinant plasmid was identified by double-enzyme digestion and named as pET15b-PEP-1. pET15b-PEP-1 and pGEM-T-CAT were further digested by Xho I/BamH I and Sal I/BglⅡ, respectively. The purified linear fragment of pET15b-PEP-1 and CAT cDNA fragment were ligated using two pairs of isocaudarners possessing different recognition sequences but producing compatible cohesive ends. The clone with the expected insert was selected using Xho I restriction analysis followed by sequence analysis. The recombinant plasmid was transformed into E.coli BL21(DE3) which was induced by IPTG. The recombinant protein possessed an N-terminal His-tag sequence which could be used to purify the target protein by affinity chromatography on a Ni2+-NTA-resin column. The fusion protein PEP-1-CAT was produced and confirmed by specific enzyme activity in vitro. Results Sequence analysis showed that the PEP-1 and the human CAT cDNA sequence of pET15b- PEP-1-CAT had identical sequence with designed PEP-1 peptide and human catalase cDNA sequence in GenBank (accession No. AY028632), respectively. SDS-PAGE and Western blotting confirmed successful expression and purification of PEP-1-CAT fusion protein with specific activity of 77.15 U/g. Conclusion The prokaryotic expression plasmid pET15b-PEP-1-CAT has been constructed successfully, and the successful expression and purification of PEP-1-CAT provides a basis for prevention and therapy of various disorders related to oxidative stress.  Related Articles | Metrics | Comments（0）

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Effects of radiosensitivity and X-ray dose on miR-7 expression in nasopharyngeal carcinoma  CHEN Zhi-xian, SUN Ai-min, CHEN Yong, LIU Ying, ZHAN Jun-fang, CHEN Long-hua, YUAN Ya-wei Journal of Southern Medical University    2010, 30 (08): 1810-1816.   Abstract （5358）      PDF（pc） （1103KB）（4490）       Knowledge map    Save Reference | Related Articles | Metrics | Comments（0）

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Comparison of several silver staining methods for DNA polyacrylamide gel electrophoresis LI Xi-ping, QIAN Xin-hua, YAO Ying-min, LIU Zhi-jie, LIU Yang Journal of Southern Medical University    2004, 24 (09): 1072-1074.   Abstract （5354）      PDF（pc） （304KB）（5549）       Knowledge map    Save Objective To compare 4 silver staining methods for DNA detection in polyacrylamide gel electrophoresis. Methods After the electrophoresis was completed, the gels were stained separately by four different methods, namely using ammonia-silver-citric acid, low-concentration silver nitrate, 0.1% and 0.2% silver nitrate. Results DNA was detected only by staining with 0.1% and 0.2% silver nitrate. Conclusion 0.2% silver nitrate used along with sodium hydroxide shows the highest sensitivity in DNA detection, while its use with sodium carbonate produces the best quality of the image. Reference | Related Articles | Metrics | Comments（0）

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Cloning and expression of the HER2 gene in MCF-7 cells LI Rong, ZHENG Hang, LUO Rong-cheng Journal of Southern Medical University    2005, 25 (10): 1264-1267.   Abstract （5322）      PDF（pc） （1008KB）（3696）       Knowledge map    Save Objective To clone human epidermal growth factor receptor (HER2) gene and establish a MCF-7 cell line with stable co-expression of HER2 and estrogen receptor (ER). Methods The full-length HER2 gene fragment was amplified by RT-PCR from the total RNA extracted from human breast cancer cell line SKBR-3 which over-expressed HER2 protein. The gene fragment was then inserted into the vector pcDNA3.1 to construct the recombinant expression plasmid pcDNA3.1- HER2, which was identified by sequence analysis and transfected into ER-expressing MCF-7 cells via lipofectamine. The positive cell clones were obtained after G418 selection. The MCF-7 cells transfected with HER2-cDNA was assayed by immunocytochemistry, flow cytometry and Western blotting. Results DNA sequencing results showed that HER2 gene was exactly consistent with the sequence reported in GenBank. The MCF-7 cells tansfected with HER2 could highly express HER2 protein as shown by immunocytochemistry, flow cytometry and Western blotting. Conclusion HER2 gene has been cloned successfully and the MCF-7 cell line co-expressing HER2 and ER is obtained. Reference | Related Articles | Metrics | Comments（0）

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1 Journal of Southern Medical University    2005, 25 (08): 1047-1048.   Abstract （5271）      PDF（pc） （536KB）（3331）       Knowledge map    Save Reference | Related Articles | Metrics | Comments（0）

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Prevalence of internet addiction and the related factors in middle school students in Guangzhou  XIE Yong-biao, ZHOU Ping, XU Li-ping, PENG Zi-wen Journal of Southern Medical University    2010, 30 (08): 1801-1804.   Abstract （5264）      PDF（pc） （1019KB）（4409）       Knowledge map    Save Reference | Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2012, 32 (08): 1098-.   Abstract （5231）      PDF（pc） （3386KB）（5615）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Establishment of a bladder cancer cell line with toll-like receptor 2 gene knockdown and identification of its biological characteristics  WEI Jian-an, ZENG Xing, HAN Ling, HUANG Yu Journal of Southern Medical University    2010, 30 (08): 1797-1800.   Abstract （5189）      PDF（pc） （1128KB）（4456）       Knowledge map    Save Reference | Related Articles | Metrics | Comments（0）