Difference in HLA-A*02 allele distribution between Han populations in south and north China

Abstract

Abstract: Objective To investigate the distributions of HLA-A∗02 alleles in Han populations and compare their difference between the south and north in China.Methods A total of 208 individuals from south China and 109 from north China were randomly selected from registered bone marrow donors in Chinese Han population, who were tested positive for HLA-A*02 alleles by PCR with sequence-specific primers (PCR-SSP). Genotyping of the alleles was performed using PCR-sequence-based typing (PCR-SBT).Results Six different HLA-A∗02 alleles (A∗020101, A∗0203, A∗0205, A∗0206, A∗0207 and A∗0210) were identified in the two Chinese Han populations, of which A∗0207(37%) was the predominant allele in southerners and A∗020101(48%) in the northerners. A∗020101(31%), A∗0203(16%) and A∗0206(14%) were common alleles in the southerners in comparison with ∗#8727;0206(21%) and A∗0207(23%) alleles in the northerners. The overall distribution of A∗02 alleles and the frequencies of A∗020101, A∗0203 and A∗0207 in the two populations differed significantly. The heterozygosity of A∗02 in the southerners and northerners was higher than 90% and 80% at the high- and low-resolution levels, respectively, and the distribution of A∗02 homozygote at low-resolution level in both populations showed diversity and regularity at high-resolution level.Conclusions HLA-A∗02 alleles possess high heterogeneity and genetic diversity in Chinese Han population with significantly different distributions in the two populations. HLA-A∗020101, A∗0203 and A∗0207 may serve as the genetic markers for dividing Chinese Han individuals into southerners and northerners in anthropological studies.

CLC Number:

CHENG Liang-hong, JIN Shi-zheng, GAO Su-qing, LI Zhen, ZOU Hong-yan, WANG Da-ming, WU Guo-guang. Difference in HLA-A*02 allele distribution between Han populations in south and north China[J]. Journal of Southern Medical University, 2005, 25(03): 321-324.

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