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Effects of all-trans-retinoic acid, acitretin and tazarotene on apoptosis and Bax/Bcl-2 expressions of human melanoma cells A375 and the significance RAN Li-wei, TAN Sheng-shun, WANG Wan-juan, QIU Shi, LEI Xiao-bing, ZENG Wei-hui Journal of Southern Medical University    2005, 25 (08): 972-974,978.   Abstract （1560）      PDF（pc） （872KB）（155093）       Knowledge map    Save Objective To investigate the effects of all-trans retinoic acid (ATRA), acitretin and tazarotene on apoptosis and Bax/Bcl-2 protein expressions of human melanoma A375 cells. Methods The effects of retinoids on apoptosis and Bax/Bcl-2 protein expressions of A375 cells in vitro were examined. Apoptosis analysis with double staining with annexin V-FITC and PI was performed using flow cytometer. SABC immunocytochemistry was employed for detection of Bax/Bcl-2 protein expressions. Results At the concentration of 1×10-5 mol/L, ATRA, acitretin and tazarotene all induced apoptosis of A375 cells with apoptosis ratio of 5.03% (P<0.05), 13.42% (P<0.05) and 2.88% (P>0.05), respectively, and acitretin induced more significant apoptosis than the other two agents (P<0.05). In addition, all the three agents significantly increased the number of cells positive for Bax expression and decreased the number of cells expressing Bcl-2 (P<0.05), among which acitretin had the strongest effects. Conclusions ATRA, acitretin and tazarotene mediate apoptosis of A375 cells possibly through, at least partially, the mitochondrial pathway. Acitretin may be utilized as a valuable alternative for treating melanoma. Reference | Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2012, 32 (12): 1777-.   Abstract （4021）      PDF（pc） （1161KB）（17478）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (10): 1687-.   Abstract （4311）      PDF（pc） （3273KB）（10839）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2007, 27 (11): 1699-.   Abstract （2837）      PDF（pc） （172KB）（9614）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2006, 26 (04): 405-405.   Abstract （3527）      PDF（pc） （6562KB）（8708）       Knowledge map    Save Reference | Related Articles | Metrics | Comments（0）

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Biochemical and pathological analysis of mice with type 2 diabetes mellitus induced by high-fat diet and low-dose streptozotocin injections Journal of Southern Medical University    2014, 34 (08): 1115-.   Abstract （4094）      PDF（pc） （697KB）（8253）       Knowledge map    Save Objective To analyze the biochemical and pathological changes in mice with type 2 diabetes mellitus (T2DM) induced by high-fat diet combined with low-dose streptozotocin (STZ) injections. Methods C57BL/6J mice were divided randomly into normal control group (NC group), high-fat diet group (HC group) and high-fat diet plus STZ group (HC+STZ group). The mice were fed on normal chow or a high-fat diet for 1 month before two introperitoneal injections of STZ (40 mg/ kg) or citrate buffer with an interval of 24 h as appropriate. Fasting blood glucose (FBG) was detected every week for 4 weeks, and oral glucose tolerance test (OGTT) was performed one month after the injections, after which the biochemical profiles, islet and liver were evaluated by immunohistochemical and pathological analysis. Results In HC+STZ group, FBG was above the cutoff value (13.89 mmol/L) in 75% of the mice at 1 week after STZ injections and in all the mice at two weeks except for the death of 1 mouse, with a success rate of modeling of 91.3%. FBG in HC group, though slightly higher than that in NC group, remained normal (6.8 mmol/L). The body weight in HC+STZ and HC groups was significantly higher than that in NC group after feeding but without obvious increases after the injections (P<0.01). Blood glucose in HC+STZ group at 0.5 to 2 h after OGTT and the area under curve (AUC) were higher than those in NC and HC groups (P<0.01); the AUC in HC group was a also higher than that in NC group (P<0.05). Plasma creatinine was significantly higher in HC+STZ group than in NC (P<0.01) and HC (P<0.05) groups. Insulin secretion by the islets decreased obviously in HC+STZ and HC group. The mice in HC+STZ group showed atrophy, fibrosis, and vacuolization in the islets with mild fatty liver but no visible renal pathologies. Conclusion High-fat diet and low-dose STZ injections can induce T2DM in mice with very similar biochemical and pathological changes to human T2DM and with such complications as fatty liver. Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (02): 234-.   Abstract （3858）      PDF（pc） （1404KB）（7956）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (04): 572-.   Abstract （6263）      PDF（pc） （1364KB）（7805）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Screening of short peptides binding to cell surface interleukin-2 receptor α chain LIU Bei-yi, ZHU Ping, LUO Hai-bo, FU Ning Department of Immunology, Southern Medical University, Guangzhou 510515, China Journal of Southern Medical University    2006, 26 (07): 971-974.   Abstract （2973）      PDF（pc） （339KB）（7701）       Knowledge map    Save Objective To screen and characterize the short peptides which bind specifically to interleukin-2 (IL-2) receptor α chain (IL-2Rα) for acquisition of small antagonists for blocking the binding of IL-2 with IL-2Rα. Methods 12-mer phage displayed peptide library was screened with the target cells of MT-2 cells which expressed IL-2Rα at high levels. The binding phage clones were eluted by anti-IL-2Rα monoclonal antibody. After 3 rounds of screening, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences of the positive clones were deduced from the DNA sequences. Results Seven positive clones were screened out of the 17 phage clones bound to MT-2 cells. The positive clone M15 could bind specifically to MT-2 cell and PHA-activated peripheral blood monouclear cells. Amino acid sequence analysis identified 6 sequences, all of which contained hydrophilic residues, and 5 of these 6 sequences included Tyr, Phe and Leu conservative residues. Conclusion The peptide sequences containing Tyr, Phe conservative residues identified in this study can bind to cell surface IL-2Rα.  Related Articles | Metrics | Comments（0）

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Prenatal diagnosis of single umbilical artery: implications for chromosomal abnormalities and neonatal outcome Journal of Southern Medical University    2013, 33 (03): 451-.   Abstract （4100）      PDF（pc） （1156KB）（7424）       Knowledge map    Save Objective To investigate the implications of a prenatal diagnosis of single umbilical artery (SUA) for chromosomal abnormalities and neonatal outcomes. Methods From January, 2008 to June, 2012, color Doppler ultrasound identified 44 fetuses with SUA. Prenatal diagnoses with amniocentesis or umbilical blood sampling were subsequently ordered for routine chromosome karyotyping and the newborns were followed up for assessing the neonatal outcomes. Results Of all the 44 fetuses, 24 had uncomplicated SUA, and 20 had other concurrent abnormalities (including 8 with abnormal ultrasound soft indexes and 12 with chromosomal abnormalities). The two groups of fetuses showed significant differences in gestational weeks at delivery and incidence of chromosomal abnormalities but not in neonatal weight, placenta weight or APGAR score. Conclusions Fetuses with a prenatal diagnosis of SUA and other development abnormities need to undergo prenatal chromosomal examination. For fetuses with uncomplicated SUA, careful ultrasound examination is necessary to avoid missed diagnosis of potential congenital abnormalities. Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2010, 30 (11): 2533-.   Abstract （1694）      PDF（pc） （1135KB）（7154）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (07): 1220-.   Abstract （7051）      PDF（pc） （1288KB）（7061）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Impact of Macao Medical Voucher Program on health outcomes of the residents: changes of mortality from circulatory system diseases Journal of Southern Medical University    2014, 34 (08): 1125-.   Abstract （3316）      PDF（pc） （494KB）（7053）       Knowledge map    Save Objective To examine whether the implementation of Macao Medical Voucher Program has helped promote the health outcomes of the residents in the case of mortality from circulatory system diseases. Methods Based on 144 monthly observations of the mortality from circulatory system diseases in Macao during 2001-2012, we carried out a trend analysis of the time series to identify significant differences in the mortality data after the implementation of the Medical Voucher in Macao. This study was controlled for the compounding factors including medical resources (numbers of physicians, nurses and patient beds per thousand population and public healthcare expenditure), economic development level (GDP per capita), social human development level, population aging factor, natural seasonal effects and long-term trends. Results During 2010-2012 when the Medical Voucher Program in Macao was implemented, the annual mortality rates from circulatory system diseases were significantly lowered by 24% as compared with those recorded during 2001-2009 (P<0.01), which was equivalent to avoiding 123 deaths related of circulatory system diseases per year. Conclusion Evidence in this study suggests a robust connection between the timing of the implementation of Macao Medical Voucher Program and a significant decrease in the mortality from circulatory system diseases in Macao, but their causal relationship awaits confirmation in further research. Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2009, 29 (06): 1312-.   Abstract （3379）      PDF（pc） （919KB）（7049）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2010, 30 (01): 153-.   Abstract （2948）      PDF（pc） （1024KB）（7034）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2009, 29 (04): 791-.   Abstract （3078）      PDF（pc） （1770KB）（7006）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (07): 1114-.   Abstract （4415）      PDF（pc） （1353KB）（6969）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2011, 31 (03): 443-.   Abstract （3402）      PDF（pc） （1905KB）（6833）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Fluorescence quantification of intracellular calcium of rat smooth muscle cells WANG Shui-yun1, YAN Fang2, PENG He-xin1, ZHENG Chang-dong3, BAI Cheng-wen1 Journal of Southern Medical University    2004, 24 (04): 450-452.   Abstract （3367）      PDF（pc） （217KB）（6729）       Knowledge map    Save Objective To explore the kinetic changes of calcium in rat smooth muscle cells and establish a method for quantification of intracellular calcium. Methods Rat mesenteric arteriolar smooth muscle cells (ASMCs) were isolated and the kinetic changes of calcium were measured using highly sensitive Ca2+ fluorescent probe indo-1 with laser scanning confocal microscopy (LSCM). The dissociation constant values (Kd) of the fluorescent probe indo-1 was measured at 37 ℃, and according to the conversion formula from fluorescence intensity to concentration, the concentration of Ca2+ was calculated. Results Analysis of the fluorescent images using LSCM showed that[Ca2+]i in the ASMCs were significantly elevated in response to stimulation with dexamethasone, and spontaneous calcium waves as well as intracellular calcium overloading were observed occasionally. Conclusion Fluorescence quantification with LSCM is applicable for detecting the kinetic changes of intracellular[Ca2+]i. Reference | Related Articles | Metrics | Comments（0）

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Journal of Southern Medical University    2008, 28 (05): 764-.   Abstract （2839）      PDF（pc） （300KB）（6438）       Knowledge map    Save Related Articles | Metrics | Comments（0）