Journal of Southern Medical University ›› 2012, Vol. 32 ›› Issue (12): 1683-.

    Next Articles

Comparative study of retinopathy in C57BL/6and eNOS-knockout mouse models of type 1diabetes mellitus

  

  • Online:2012-12-20 Published:2012-12-20

Abstract: ObjectiveTo compare the retinal function and retinal vascular pathologies in C57BL/6and eNOS-knockout (eNOS-/-)
mouse models of type1diabetes mellitus (T1DM) induced by streptozotocin (STZ).MethodsT1DM models were established
in 6- to 8-week-old C57BL/6 and eNOS-/-mice by intraperitoneal STZ injection. Electroretinogram (ERG) examination,
fluorescein angiography (FFA), immunofluorescence staining and retinal ganglion cell counts were carried out before and after
STZ injection.ResultsDiabetic C57BL/6and eNOS-/-mice showed significantly lowered a-wave and b-wave amplitude in ERG
and reduced number of retinal ganglion cells (P<0.05), and the retinal vessels in diabetic eNOS-/-mice became tortuous.
Compared with diabetic C57BL/6mice, diabetic eNOS-/-mice showed more severe pathological changes in retinal function and
retinal vessels with also more rapid onset of pathologies.ConclusionCompared with C57BL/6mouse models, eNOS-/-mouse
models of T1DM can better represent the occurrence and development of diabetic retinopathy, thus providing an ideal model
for diabetes and diabetic retinopathy studies.