Expression of nestin, an intermediate filament protein, in human fetal hepatic stem cells

Abstract

Abstract: Objective To explore a new oval cell marker by examining the expression of nestin in human fetal hepatic stem cells in various stages during human fetal development. Methods Human fetal tissue samples of various developmental stages were obtained and fixed for preparation of paraffin sections. The expression of nestin, generally recognized as a specific marker for neural precursor cells, in fetal hepatic stem cells was detected using immunocytochemical staining (ABC method). Results Nestin-positive oval cells, characterized to be hepatic stem cells, were found in the limiting plate, which arranged tightly in monolayer to form ductules that encapsulated the early-stage portal area in a sheath-like fashion, and also partially encapsulated the primary portal area. With the development of the secondary portal areas, the nestin-positive oval cells gradually centered around the Hering canal. In addition, some nestin-positive mononuclear cells were found scattering in the hepatic cords and sinusoids, especially in the mesenchymal tissue of the portal areas. Conclusion Nestin-positive oval cells exist in human fetal liver and their origin and function await further investigation.

CLC Number:

R321.5

SUN Xiao-yan, AN Jing. Expression of nestin, an intermediate filament protein, in human fetal hepatic stem cells[J]. Journal of Southern Medical University, 2004, 24(02): 207-209.

References

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