Apoptosis and regulation of expressions of apoptosis-related gene Bcl-2 and p53 induced by selenium dioxide in three leukemia cell lines

Abstract

Abstract: Objective To investigate the mechanisms underlying the effect of selenium dioxide (SeO₂) on the proliferation,apoptosis,and apoptosis-related gene expressions of Bcl-2 and p53 in 3 leukemia cell lines NB4,K562 and HL-60.Methods The three leukemia cell lines were treated with 3,10 and 30 mmol/L SeO₂ and apoptosis detected by flow cytometry and analysis of p53 and Bcl-2 expressions. Results SeO₂ at 10 and 30 mmol/L could inhibit the proliferation of three leukemia cell lines. SeO₂ treatment at 30 mmol/L for 48 h induced an apoptosis rate of 54.0 %,46.5 %,49.6 % in NB4,K562,and HL-60 cells respectively,and down-regulated Bcl-2 expression in NB4 and K562 but not in HL-60 cells.Conclusion SeO₂ can induce apoptosis in NB4,K562 and HL-60 leukemia cells,involving the down-regulation of Bcl-2 and up-regulation of p53.

CLC Number:

R733.7
R39.2

WANG Xiao-hua¹, WEI Ya-ming², BAI Hai³, OU Jian-feng³, LU Ji-hong³, ZHENG Rong-liang⁴. Apoptosis and regulation of expressions of apoptosis-related gene Bcl-2 and p53 induced by selenium dioxide in three leukemia cell lines[J]. Journal of Southern Medical University, 2004, 24(10): 1160-1163.

References

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