Abstract: Objective To describe a modified surgical approach to produce rat ischemia-reperfusion injury model with intraluminal suture.Methods After exposure and ligation of the common carotid artery (CCA),the left external carotid artery and pterygopalatine artery were opened in which a 3-0 nylon suture was introduced intraluminally from the distal end of the ligature of the CCA using a scalp needle. Reperfusion injury was induced by withdrawal of the suture. Results The length of the suture was about 20.0±1.8 mm and the success rate of model establishment was nearly 70%. The rats developed typical symptoms and pathological manifestations after the surgery.Conclusion This modified surgical approach is simple for model establishment and does not require special microsurgical skills to ensure the high success rate.