Effects of catalase on the mitochondria and apoptosis of SW480 cells

Abstract

Abstract: Objective To investigate the effects of catalase on the mitochondria and apoptotic behavior of SW480 cells treated by lipopolysaccharide (LPS), and the changes in intracellular expression of nuclear factor κB (NF-κB). Methods LPS-stimulated SW480 cells were treated with catalase and the changes in their mitochondria and apoptotic behavior were observed by transmission electron microscopy. The expression of NF-κB was detected by immunohistochemical method. Results Mitochondria damages and apoptosis were slightly diminished in the LPS-stimulated cells with catalase pretreatment, and the NF-κB expression was also decreased. Conclusion Catalase protect the mitochondria of SW480 cells from damages by LPS and inhibit the cell apoptosis, possibly due to the activation of NF-κB.

CLC Number:

Q255
Q554.6

WANG Qun-ying, WANG Ji-de, ZHONG Shi-shun, WANG Ya-dong, SUN Yong, LI Liang-ren ZHANG Ya-li. Effects of catalase on the mitochondria and apoptosis of SW480 cells[J]. Journal of Southern Medical University, 2004, 24(12): 1419-1421.

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