Killer immunoglobulin-like receptor gene distribution in Guangdong Han population

Abstract

Abstract: Objective To investigate the distribution of killer immunoglobulin-like receptor (KIR) gene in Guangdong Han population. Methods KIR phenotype was examined by PCR with sequence-specific primers in 96 subjects of Han nationality in Guangdong Province of China, and KIR frequency was calculated and compared with those in Caucasian, north Indian and Japanese populations. Result The gene expression frequency of KIR in Guangdong Han people was 2DL1(0.85), 2DL2(0.12), 2DL3(0.58), 2DL4(1), 2DL5(0.24), 3DL1(0.96), 3DL2(1), 3DL3(1), 2DP1(0.97), 2DP2(0.98), 2DS1(0.10), 2DS2(0.30), 2DS3(0.02), 2DS4(0.28), 1D(0.65), 2DS5(0.19), and 3DS1(0.23) respectively. Comparison of the KIR recognizing the same HLA ligand suggested significantly higher expression frequency of inhibitory KIR than that of activating KIR. Compared with Caucasian and north Indian populations, Guangdong Han population had significantly lower expression frequency of activating KIR gene with the exception of KIR2DS4. Conclusion Different KIR genes have different expression frequencies in Guangdong Han population, and KIR gene distribution varies between populations of different races.

CLC Number:

YIN Xiao-lin¹, GUO Kun-yuan¹, MA Hong-jing¹, SHANG Zheng-chuan¹, XIAO Lu-lu², YE Xin², ZHAO Yang². Killer immunoglobulin-like receptor gene distribution in Guangdong Han population[J]. Journal of Southern Medical University, 2004, 24(12): 1416-1418.

References

[1] Hsu KC, Liu XR, Selvakumar A, et al. Killer Ig-like receptor haplotype analysis by gene content: evidence for genomic diversity with a minimum of six basic framework haplotypes, each with multiple subsets[J]. J Immunol, 2002, 169:5118-29.
[2] Uhrberg M, Valiante NM, Shum BP, et al. Human diversity in killer cell inhibitory receptor genes[J]. Immunity, 1997, 7: 753-63.
[3] Rajalingam R, Krausa P, Shilling HG, et al. Distinctive KIR and HLA diversity in a panel of north Indian Hindus [J]. Immunogen,2002, 53: 1009-19.
[4] Yawata M, Yawata N, Mcqueen KL, et al. Predominance of group A KIR haplotypes in Japanese associated with diverse NK cell repertoires of KIR expression [J]. Immunogen, 2002, 54: 543-50.
[5] Vilches C, Parham P. KIR: diverse, rapidly evolving receptors of innate and adaptive immunity [J]. Annu Rev Immunol, 2002, 20:217-51.
[6] Yen JH, Moore BE, Nakajima T, et al. Major histocompatibility complex class I-recognizing receptors are disease risk genes in rheumatoid arthritis[J]. J Exp Med, 2001, 193(10): 1159-67.
[7] Martin MP, Gao X, Lee JH, et al. Epistatic interaction between KIR3DS1 and HLA-B delays the progression to AIDS [J]. Nat Genet, 2002, 31 (4): 429-34.
[8] Guerra N, Guillard M, Angevin E, et al. Killer inhibitory receptor(CD158b) modulates the lytic activity of tumor-specific T lymphocytes infiltrating renal cell carcinomas [J]. Blood, 2000, 95(9):2883-9.
[9] Ruggeri L, Capanni M, Urbani E, et al. Effectiveness of donor natural killer cell alloreactivity in mismatched hematopoietic transplants[J]. Science, 2002, 295(5562): 2097-100.
[10] Martin MP, Nelson G, Lee JH, et al. Cutting edge: susceptibility to psoriatic arthritis: influence of activating killer Ig-like receptor genes in the absence of specific HLA-C alleles [J]. J Immunol,2002, 169(6): 2818-22.