Journal of Southern Medical University ›› 2025, Vol. 45 ›› Issue (5): 893-900.doi: 10.12122/j.issn.1673-4254.2025.05.01
Lihua ZHOU(), Xun ZHANG, Yingying YU, Panpan ZHANG
Received:
2024-11-01
Online:
2025-05-20
Published:
2025-05-23
Contact:
Lihua ZHOU
E-mail:zhoulihuaby@sina.com
Supported by:
Lihua ZHOU, Xun ZHANG, Yingying YU, Panpan ZHANG. Role of the Nrf2/HO-1 pathway in cypermethrin-induced oxidative injury of mice hippocampal neurons[J]. Journal of Southern Medical University, 2025, 45(5): 893-900.
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URL: https://www.j-smu.com/EN/10.12122/j.issn.1673-4254.2025.05.01
Gene | Serial number | Primer sequences (5'-3') | Product size |
---|---|---|---|
Nrf2 | NM_010902.4 | F: AGTCGCTTGCCCTGGATATC R: GAACAGCGGTAGTATCAGCCA | 216 bp |
HO-1 | NM_010442.2 | F: GCTAAGACCGCCTTCCTGCT R: ACGAAGTGACGCCATCTGTGA | 105 bp |
β-actin | NM_007393.3 | F: GTGACGTTGACATCCGTAAAGA R: GTAACAGTCCGCCTAGAAGCAC | 287 bp |
Tab.1 Primers for RT-qPCR amplification of the target genes
Gene | Serial number | Primer sequences (5'-3') | Product size |
---|---|---|---|
Nrf2 | NM_010902.4 | F: AGTCGCTTGCCCTGGATATC R: GAACAGCGGTAGTATCAGCCA | 216 bp |
HO-1 | NM_010442.2 | F: GCTAAGACCGCCTTCCTGCT R: ACGAAGTGACGCCATCTGTGA | 105 bp |
β-actin | NM_007393.3 | F: GTGACGTTGACATCCGTAAAGA R: GTAACAGTCCGCCTAGAAGCAC | 287 bp |
Fig.3 HE staining showing morphological alterations of the hippocampal neurons in control and CYP-treated mice. I: Molecular layer; II: Pyramidal layer in CA1 and CA3 regions (granular layer in DG); III: Polymorphic layer. black arrows: Chromatin condensation.
Fig.6 Nrf2 and HO-1 protein expressions in mice hippocampus examined by Western blotting. A: Protein bands of Nrf2 and HO-1. B: Bar chart of gray values analyze of the protein bands (n=3). **P<0.01 vs Control; #P<0.05 vs CYP 5 mg/kg.
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