Abstract: Objective To investigate the effect of BIX01294 (BIX), a methyltransferase inhibitor, on the migration and decidualization of the stromal cells in mouse uterus. Methods Mouse endometrial stromal cells were isolated and cultured from the uterus of pregnant mice on day 3.5 of gestation. The migration and decidualization of mouse endometrial stromal cells treated with BIX at different concentrations were observed with wound healing assay and real-time PCR. Results The migration distance of mouse endometrial stromal cells increased as the BIX concentration increased within the range below 15 μmol/L. Compared with the control cells, the cells treated with BIX (15 μmol/L) showed significantly increased migration distances, but increasing BIX concentration to 20 μmol/L did not further increase the cell migration distance and began to cause cell death. Compared with the control cells, the BIX-treated stromal cells exhibited significantly down-regulated expression of Ehmt2 mRNA, and 15 μmol/L BIX caused inhibition of decidualization in the stromal cells. Conclusion Within a defined concentration range, BIX promotes the migration and inhibits decidualization of mouse uterine stromal cells by inhibiting the expression of Ehmt2 mRNA.