Abstract: Objective To investigate whether vitamin C can promote the proliferation ability of bone marrow mesenchymal
stem cells (BMMSCs) derived from aging mice. Methods The senescence-accelerated mouse prone 6 (SAMP6) mice and
senescence-accelerated mouse resistant 1 (SAMR1) mice were used as the test group and the control group, respectively, and
the SAMP6 mice were examined by micro-CT to verify the senescent phenotype. BMMSCs were harvested from the two mouse
lines and cultured in vitro, and the cells from SAMP6 mice were subjected to treatment with different concentrations of vitamin
C. The proliferation ability of the cells from the two mouse lines was tested using MTT assay and growth curves, and
TeloTAGGG Telomerase PCR ELISA was used to measure the telomerase activity; PCR and Western blotting were performed
to detect the expression level of telomerase reverse transcriptase (TERT) in the cells. Results The SAMP6 mice displayed a
bone senescent phenotype. The proliferation ability of BMMSCs derived from SAMP6 mice and their telomerase activity were
significantly lower than those derived from SAMR1 mice (P<0.05). Vitamin C treatment significantly enhanced the
proliferation ability of BMMSCs derived from SAMP6 mice in a dose-dependent manner (P<0.05) and increased telomerase
activity and TERT expression in the cells (P<0.05). At the concentration of 100 μg/mL, vitamin C produced the strongest effect
in promoting the proliferation of BMMSCs from SAMP6 mice, while at the concentration of 1000 μg/ml, growth suppression
occurred in the cells. Conclusion Vitamin C can promote the proliferation of BMMSCs from aging mice possibly by increasing
the cellular telomerase activity.