Abstract: Objective To express solute carrier 26A proteins in HEK-293 cells and explore their functions. Methods
SLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing
SLC26A proteins was measured by whole-cell patch recording. Results All the SLC26A transporters were expressed on the
membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which
represented their binding capability with anions. Conclusion The SLC26A transporters expressed on HEK cells show similar
functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A
transporters.