Abstract: Objective To obtain highly efficient small interfering RNAs (siRNAs) against bone morphogenetic protein-7 (BMP-7)
for future functional analysis of BMP-7 in bone homeostasis. Methods We designed 4 independent siRNA sequences against
rat BMP-7 and transfected them in rat umbilical vein endothelial cells. After 72 h, we examined BMP-7 mRNA and protein
level in the transfected cells using real-time PCR and Western blotting, respectively. Results All the 4 siRNAs effectively
reduced BMP-7 expression in rat umbilical vein endothelial cells, and among them BMP-7-3 siRNA showed the highest
efficiency. Conclusion We have obtained an efficient siRNA to knockdown BMP-7 expression in vitro for further investigation
of the role of BMP-7 in bone and cartilage formation.