Abstract: Objective To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to
suppress the invasiveness of hepatocellular carcinoma. Methods HepG2 cells cultured in the serum of rats fed with Biejiajian
Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with
luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR. Results Biejiajian Pills-treated
sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and
lowered the activities of β-catenin/TCF4 complex. Conclusion Biejiajian Pills may serve as a potential anti-tumor agent, whose
effect might be mediated by inhibiting the Wnt/β -catenin pathway.