Abstract: Objective To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric
cancer cells. Methods We used 3’RACE to capture the APA sites of two tumor-related genes (HSP90α and SEC11A) in gastric
cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database. Results
We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different
3’UTRs. Conclusions There are new mRNA isoforms of HSP90α and SEC11A derived from ATA in gastric cancer cells, which
provides new insights into the mechanisms of gastric tumorigenesis.