Abstract: Objective To investigate the effect of PIK3CA/siRNA chitosan nanoparticle on the invasiveness of gastric carcinoma
and the potential value of PIK3CA/siRNA chitosan nanoparticle in suppressing the metastasis of gastric carcinoma. Methods
Gastric cancer cells were treated with PIK3CA/siRNA nanoparticle (with a diameter of 350 nm), and the efficiency of PIK3CA
gene interference was evaluated using Western blotting and real-time PCR. The changes of the invasive capacity of the treated
cells was assessed with Transwell assay. Results PIK3CA/siRNA chitosan nanoparticle efficiently lowered the expression level
of PIK3CA and significantly decreased the invasion of BGC823 cells. Conclusion PIK3CA gene interference mediated by
PIK3CA/siRNA chitosan nanoparticle can decrease the invasive capacity of gastric cancer cells in vitro.