Journal of Southern Medical University ›› 2014, Vol. 34 ›› Issue (10): 1420-.
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Abstract: Objective To clone and identify olfactory receptor odorant receptor 7 (OR7) gene of Aedes albopictus and analyze itsexpression profile and calcium regulation function. Method RT-PCR was used to amplify the olfactory receptor OR7 gene ofAe. albopictus and OR7 expression was detected in different tissues and organs. The coding sequence of OR7 gene was clonedin eukaryotic expression vector pME18s, which was then transfected into HEK293 cells. The calcium callback function inresponse to odor molecule stimulation was analyzed by calcium imaging technique. Results The OR7 gene of Ae. albopictuswas cloned and sequence analysis showed that its coding region was 1395 bp. RT-PCR detected OR7 expression in the larvae,pupae and adult mosquitoes, especially in female mosquitos. Preliminary analysis of calcium callback function demonstratedthe specific regulation of calcium absorption by OR7 in response to odor molecule stimulation. Conclusion The OR7 gene ofAe. albopictus has been cloned successfully. OR7 is highly expressed in female mosquitos and is capable of specific recognitionof the odor molecules.
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https://www.j-smu.com/EN/Y2014/V34/I10/1420