Abstract: We used ultra-high performance liquid chromatography-mass spectrometry (UPLC/MS) for analyzing and
identifying the active components of newborn calf serum (NCS) and fetal bovine serum (FBS). The results demonstrated
significant differences in the components between NCS and FBS. FBS appeared to have more complex components than NCS,
with mass to ratios (m/z) of the substances of 498, 273 and 448. These substances in FBS may be the main active components to
support the proliferation and differentiation of cells.