Abstract: Objective To investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and
development of colorectal cancer. Methods Colorectal cancer cell lines with stable Sam68 over-expressing and low Sam68
expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using
colony formation, MTT and Transwell assays. Results SW480 and Ls174t colorectal cell lines over-expressing Sam68 showed
significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and
HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05). Conclusion The
expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.