Abstract: Objective To explore the role of SCF/c-Kit signaling in the invasion of bladder cancer T24 cells. Methods Western
blotting was used to detect the expression of c-Kit and PI3K pathway activation stimulated by stem cell factor (SCF) in T24
cells. The invasiveness of T24 cells before and after SCF stimulation and Wortmannin (aspecific PI3K inhibitor) treatment was
evaluated using Transwell invasion assay (direct and indirect counting methods). Results T24 cells expressed c-Kit protein and
showed obvious Akt phosphorylation after stimulation with SCF (1 ng/ml) for 24 h. Compared to the control group, SCF
stimulation (1 ng/ml) caused a greater number of T24 cells to migrate through the polycarbonate film (P<0.01), and this effect
was blocked by the application of Wortmannin before the stimulation. Conclusion SCF/c-Kit signaling promotes the
invasiveness of T24 cells, and this effect is mediated by the PI3K pathway.