Abstract: Objective To establish a modified rat model of liver cancer with concurrent cirrhosis for the study of carcinogenesis
characteristics and drug intervention of liver cancer. Methods Fifty male Wistar rats weighing 100-120 g were randomly
divided into normal control group (20 rats) and model group (30 rats). In the model group, the rats were subjected to
intraperitoneal injection of 50 mg/kg DEN N-diethylnitrosamine (DEN) twice a week for 4 consecutive weeks, followed then
by weekly injections for another 10 weeks. The control rats received injections of 0.1 ml saline in the same manner. At 2, 4, 8, 12,
14, and 18 weeks, 3 rats from each group were sacrificed for assessing tumor formation and liver cirrhosis. Results Liver cancer
with concurrent cirrhosis was induced successfully after 14 weeks of DEN injections. At the 14th week, 3 out of the 5 rats were
found to have cirrhosis and LC, and at the 18th week, all the 3 rats examined had cirrhosis and liver cancer. The total
carcinogenesis rate in the rats was 75% at 18 weeks with an overall mortality of 33%. Conclusion This approach to establishing
rat models of liver cancer with concurrent cirrhosis requires simple operation, shortens the time of carcinogenesis, and ensures
a high success rate of carcinogenesis and a low mortality rate. The carcinogenesis characteristics in this model are similar to
those in human.