Journal of Southern Medical University ›› 2014, Vol. 34 ›› Issue (02): 223-.
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Abstract: Objective To study the feasibility of sustaining the viable status of a liver graft in at least 96 h by extracorporealperfusion using autologous blood. Methods Eight extracorporeal porcine liver perfusions using autologous blood wereperformed, each for 96 h with hepatectomy, cold preservation, cannulation of vessels, and initiation of perfusion withnormothermic oxygenated porcine blood. The graft viability was assessed by metabolic, synthetic, hemodynamic, andhistologic parameters. Results After 96 h of normothermic, extracorporeal perfusion using autologous blood, the isolated liversmaintained normal physiological levels of pH and electrolytes with sustained hepatic protein synthesis (complement andfactor V) throughout the perfusion. Hemodynamic parameters maintained normal physiological ranges. Histologicalinspection demonstrated good preservation of the liver with a good architectural integrity. Conclusion It is possible to sustainthe viable status of a liver graft within 96 h by extracorporeal perfusion using autologous blood.
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https://www.j-smu.com/EN/Y2014/V34/I02/223