Journal of Southern Medical University ›› 2014, Vol. 34 ›› Issue (02): 218-.
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Abstract: Objective To investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) indrug-resistant glioma cells. Methods The expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 werequantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells(drug-resistant cells) using fluorescent real-time PCR and Western blotting. Results The expression levels of PTEN weresignificantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. Theexpression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin.Conclusion MiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/Akt signaling.
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https://www.j-smu.com/EN/Y2014/V34/I02/218