Journal of Southern Medical University ›› 2014, Vol. 34 ›› Issue (02): 180-.
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Abstract: Objective To investigate the correlation between infection with L-form of Helicobacter pylori (Hp-L) and theexpressions of macrophage migration inhibition factor (MIF), matrix metalloproteinase 9 (MMP9), and vascular endothelialgrowth factor (VEGF) in gastric cancer. Methods Hp-L was examined in 80 gastric carcinoma and 50 adjacent normal tissuesby Gram staining and immunohistochemical staining, and the expressions of MIF, MMP9 and VEGF were detected byimmunohistochemical staining; the expression of MIF mRNA was detected by RT-PCR and the expression of MIF, MMP9 andVEGF proteins were detected by Western blotting in 30 fresh gastric cancer tissues and the corresponding adjacent tissues.Results Of the 80 gastric carcinoma tissues, 57 (71.25% ) showed Hp-L positivity detected by both Gram staining andimmunohistochemical staining, as compared with a rate of only 14% in the adjacent normal tissues (P<0.05). The gastriccarcinoma tissues showed higher expression levels of MIF, MMP9 and VEGF proteins than the corresponding adjacent normalmucosa; the positivity MIF, MMP-9 and VEGF proteins were significantly higher in Hp-L-positive gastric carcinoma than inHp-L-negative cases (P<0.05). Positive correlations were found between Hp-L positivity and the expressions of MIF, MMP-9and VEGF (r=0.598, 0.292, 0.341, respectively, P<0.05). The 30 fresh gastric cancer tissues showed also significantly higher MIFmRNA expression and MIF, MMP-9 and VEGF protein expressions than the adjacent tissues (t=3.729, P<0.01). The expressionsof MIF and MMP-9 were also related to the clinicopathological factors including lymph node metastasis and depth of invasion(P<0.05). Conclusion Infection with L-form of Hp-L can be an important factor that contributes to the invasion and metastasisof gastric carcinoma, the mechanism of which involves up-regulated expressions of MIF, MMP-9 and VEGF.
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https://www.j-smu.com/EN/Y2014/V34/I02/180