Abstract: Objective To study the effects of 4 different methods for disinfection of simple breathing vesicles and microbial
residue. Methods The disinfection tests were divided into 4 groups: G1 group (43 cases) with 500 mg/L chlorine dioxide
spray, G2 group (28 cases) with alcohol spray, G3 group (47 cases) with 50 mg/L trichloro isocyanuric acid (TCCA) immersion,
and G4 group (46 cases) with 50 mg/L chlorine dioxide solution immersion. Afrer 30 min of dsinfection, each group was
examined by bacterial culture and colony count. The residual bacteria were identified and typed. Results The 4 methods
showed significant differences in bacterial colony count (P<0.001). The rate of bacterial residue was 0% in G1 group, 53.6% in
G2 group, 27.7% in G3 group, and 21.7% in G4 group, showing significant differences between the 4 groups (P<0.001). The
residual bacteria included antibiotic-resistant common opportunistic pathogen such as Pseudomonas aeruginosa, Acinetobacter
baumannii and Staphylococcus haemolytic. Conclusions Disinfection with 500 mg/L chlorine dioxide spray is the best for simple
breathing vesicles. Prolonged immersion in TCCA may lead to the growth of drug-resistant pathogens in the breathing vesicles.