Abstract: Objective To detect the expression of CXCL14 in human osteosarcoma cell lines and tissues and investigate its
association with the prognosis of the patients. Methods RT-PCR, enzyme-linked immunosorbnent assay (ELISA) and real-time
PCR were used to detect the expression of CXCL14 in 4 osteosarcoma cell lines and in 40 pairs of osteosarcoma tissues and
adjacent muscular tissues. CCK8 assay and colony formation assay was used to assess the effect of CXCL14 suppression
mediated by two specific siRNAs on the proliferation of U2OS osteosarcoma cells. Immunohistochemistry was performed to
detect the expression of CXCL14 in 58 osteosarcoma tissues, and Kaplan-Meier method and log-rank test were performed for
survival analysis of the patients. Results Significant up-regulation of CXCL14 expression was found in the osteosarcoma cell
lines and in osteosarcoma tissues compared with the adjacent muscles (P<0.01). In U2OS cell, suppression of CXCL14
expression by siRNA significantly inhibited the cell proliferation (P<0.01) and colony formation rate (P<0.05). Kaplan-Meier
survival analysis indicated that patients with high CXCL14 expression had worse prognosis than those with low CXCL14
expression (P=0.02). Conclusion CXCL14 is up-regulated in both osteosarcoma cell lines and primary osteosarcoma tissues to
promote the proliferation of osteosarcoma cells. A high CXCL14 expression in osteosarcoma tissues is associated with a poor
prognosis, suggesting the that CXCL14 serve as a potential therapeutic target for osteosarcoma.