Abstract: Objective To investigate the changes in the gene expression profiles in a multiple myeloma cell line after
knocking-down the expression of SNF5, a core component of the SWI/SNF chromatin remodeling complex. Methods The total
RNA were extracted from tetracycline-inducible SNF5 knockdown (KD) cell line derived from KM3 cells for gene expression
profiling by affymetrix microarray and bioinformatic analysis. Results Knockdown of SNF5 inhibited KM3 cell proliferation.
A total of 545 genes were found to be differentially expressed in the cells with SNF5 knockdown, among which 214 were
up-regulated and 331 were down-regulated. Conclusions SNF5 is essential for the growth of multiple myeloma cells and can
regulate the expression of the genes associated with cell growth and apoptosis.